ADAMTS Proteases

ADAMTS Proteins: Concepts, Challenges, and Prospects.- Inhibition of ADAMTS1 Expression by Lentiviral CRISPR/Cas9 Gene Editing Technology.- Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins using Cell-Based Assays.- Challenges and Solutions for Purification of ADAMTS Proteases: An Overview.- Purification of Native or Recombinant ADAMTS2 and Procollagen I Cleavage Assay.- ADAMTS7: Recombinant Protein Expression and Purification.- Purification and Activity Determination of ADAMTS-4 and ADAMTS-5 and Their Domain Deleted Mutants.- Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins.- Assay for Reelin-Cleaving Activity of ADAMTS and Detection of Reelin and Its Fragments in the Brain.- Chemical Modification of Proteoglycanases with Biotin.- Analysis of Aggrecanase Activity using Neoepitope Antibodies.- Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4.- Purification of Recombinant ADAMTSL2.- Expression Analysis by RNAscope(TM) In Situ Hybridization.- Analysis of ADAMTS Effects on Cell Adhesion and Migration.- Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils.- Alizarin Red and Alcian Blue Preparations to Visualize the Skeleton.- Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS Proteins.- Quantification of Extracellular Matrix Fiber Systems Related to ADAMTS Proteins.- Visualization of Perineuronal Nets in Central Nervous System Tissue Sections.- Visualization and Quantification of Pericellular Matrix.- Quantitative Mass Spectrometry-Based Secretome Analysis as a Tool to Investigate Metalloprotease and TIMP Activity.- Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates.- Mapping the N-Terminome in Tissue