Amyloid Proteins

In Vitro Assays.- Preparation of Aggregate-Free,Low Molecular Weight Amyloid? for Assembly and Toxicity Assays.- Determination of Peptide Oligomerization State Using Rapid Photochemical Crosslinking.- In Vitro Preparation of Prefibrillar Intermediates of Amyloid-? and ? Synuclein.- Purification of Recombinant Tau Protein and Preparation of Alzheimer-Paired Helical Filaments In Vitro.- Cyclic Amplification of Protein Misfolding and Aggregation.- X-Ray Diffraction Studies of Amyloid Structure.- Molecular Electron Microscopy Approaches to Elucidating the Mechanisms of Protein Fibrillogenesis.- Time -Lapse Atomic Force Microscopy in the Characterization of Amyloid-Like Fibril Assembly and Oligomeric Intermediates.- Fourier Transform Infrared and Circular Dichroism Spectroscopies for Amyloid Studies.- Quasielastic Light Scattering for Protein Assembly Studies.- Intrinsic Fluorescent Detection of Tau Conformation and Aggregation.- Quantitative Measurement of Fibrillogenesis by Mass Spectrometry.- Cell Culture Assays.- Isolation and Culturing of Human Vascular Smooth Muscle Cells.- Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy.- Purification of Human Wild-Type or Variant Cystatin C From Conditioned Media of Transfected Cells.- Prion Propagation in Cell Culture.- In Vivo-Related Assays.- Preparation and Propagation of Amyloid-Enhancing Factor.- Purification of Amyloid Protein AA Subspecies From Amyloid-Rich Human Tissues.- Purification of Transthyretin and Transthyretin Fragments From Amyloid-Rich Human Tissues.- Extraction and Chemical Characterization of Tissue-Deposited Proteins From Minute Diagnostic Biopsy Specimens.- Tissue Processing Prior to Protein Analysis and Amyloid-ß Quantitation.- ELISA Method for Measurement ofAmyloid-ß Levels.- Histological Staining of Amyloid-ß in Mouse Brains.- The Mouse Model for Scrapie.- Radiolabeling of Amyloid-ß Peptides.- In Vivo Imaging of Amyloid-ß Deposits in Mouse Brain With Multiphoton