Auditory and Vestibular Research
Methods and Protocols
(Sprache: Englisch)
This volume describes current RNA, protein, and imaging protocols that have provided insights into genetic regulation as well as a greater understanding of the genes and pathogens involved in diseases of the ear.
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Produktinformationen zu „Auditory and Vestibular Research “
This volume describes current RNA, protein, and imaging protocols that have provided insights into genetic regulation as well as a greater understanding of the genes and pathogens involved in diseases of the ear.
Klappentext zu „Auditory and Vestibular Research “
Hearing is a sensory modality critical to both language and cognitive development. In its absence, and without sensory input through another modality, such as the manual/visual modality of sign language, cognitive and language development can be severely impaired in the earliest formative years of a child. In its endeavor to d- cover the mechanisms underlying audition, the ?eld of auditory science has provided rich comparative physiological studies, allowing insights into both the micromecha- cal and electrochemical world of this system. For many years, the auditory/vestibular sciences have been in?uenced by the discoveries of electrical engineers and sensory physiologists, who have provided insights into the functions of this dynamic system. The early discoveries in these ?elds, as well as advancements in microprocessing and materials technologies, provided a means whereby hearing could be regained partly through the use of a bionic device, known as a cochlear implant. Presently, this device and the auditory brainstem implant are the only ones to prosthetically replace brain function. With the advent of molecular biology tools, such as RT-PCR, the auditory and vestibular ?elds have made great strides in understanding the genetic basis for various hearing and balance disorders over the past ?fteen to twenty years. These technologies permitted the discovery of genes that control inner ear structure and function by ov- coming the hurdle of working with small amounts of tissue, as found in the inner ear.
Inhaltsverzeichnis zu „Auditory and Vestibular Research “
Part 1: - Nucleic Acid Protocols RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning - Casilda Trujillo-Provencio, TuShun R. Powers, David R. Sultemeier, and Elba E. Serrano
- Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arrays - Ana E. Vázquez, Liping Nie, and Ebenezer N. Yamoah
- In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections - Hakim Hiel
- Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification - Takunori Satoh and Donna M. Fekete
- Genetic Fate Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus - Jun Chul Kim and Susan M. Dymecki
- The Practical Use of Cre and loxP Technologies in Mouse Auditory Research - Yiling Yu and Jian Zuo
- Helios® Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium - Inna A. Belyantseva
- Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear - John V. Brigande, Samuel P. Gubbels, David W. Woessner, Jonathan J. Jungwirth, and Catherine S. Bresee
- Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear - Kazuo Oshima, Pascal Senn, and Stefan Heller
- Molecular Biology of Vestibular Schwannomas - Long-Sheng Chang and D. Bradley
- Welling Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis of Otitis Media Causing Pathogens - Jonathan C. Thomas and Melinda M. Pettigrew
- Fluorescence 'In situ' Hybridization for the Detection of Biofilm in the Middle Ear and Upper Respiratory Tract Mucosa - LauraNistico, Armin Gieseke, Paul Stoodley, Luanne Hall-Stoodley, Joseph E. Kerschner, and Garth D. Ehrlich
- Positional Cloning of Deafness Genes - Hannie Kremer and Frans P. M. Cremers
Part II:
- Amino Acid Protocols Twist-off Purification of Hair Bundles - Jung-Bum Shin, James Pagana, and Peter G. Gillespie
- Yeast Two-Hybrid
... mehr
Screening to Test for Protein-Protein Interactions in the Auditory System - Dhasakumar S. Navaratnam
- The Use of 2-D Gels to Identify Novel Protein-Protein Interactions in the Cochlea - Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H. A. Sokolowski
- Identification of Functionally Important Residues/Domains in Membrane Proteins Using an Evolutionary Approach Coupled with Systematic Mutational Analysis - Lavanya Rajagopalan, Fred A. Pereira, Olivier Lichtarge, and William E. Brownell
- In vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation - Margaret C. Harvey and Bernd H. A. Sokolowski
- Identification of Transcription Factor-DNA Interactions Using Chromatin Immunoprecipitation Assays - Liping Nie, Ana E. Vazquez, and Ebenezer N. Yamoah
- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Proteins in Inner-Ear Sensory Epithelia - Dennis G. Drescher, Neeliyath A. Ramakrishnan, and Marian J. Drescher
- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research - Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan
Part III:
- Imaging Protocols 22. Fluorescence Microscopy Methods in the Study of Protein Structure and Function - Heather Jensen-Smith
- The Use of 2-D Gels to Identify Novel Protein-Protein Interactions in the Cochlea - Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H. A. Sokolowski
- Identification of Functionally Important Residues/Domains in Membrane Proteins Using an Evolutionary Approach Coupled with Systematic Mutational Analysis - Lavanya Rajagopalan, Fred A. Pereira, Olivier Lichtarge, and William E. Brownell
- In vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation - Margaret C. Harvey and Bernd H. A. Sokolowski
- Identification of Transcription Factor-DNA Interactions Using Chromatin Immunoprecipitation Assays - Liping Nie, Ana E. Vazquez, and Ebenezer N. Yamoah
- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Proteins in Inner-Ear Sensory Epithelia - Dennis G. Drescher, Neeliyath A. Ramakrishnan, and Marian J. Drescher
- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research - Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan
Part III:
- Imaging Protocols 22. Fluorescence Microscopy Methods in the Study of Protein Structure and Function - Heather Jensen-Smith
... weniger
Bibliographische Angaben
- 2008, XII, 428 Seiten, 13 farbige Abbildungen, Maße: 19,8 x 26,6 cm, Gebunden, Englisch
- Herausgegeben: Bernd Sokolowski
- Verlag: Springer, Berlin
- ISBN-10: 1934115622
- ISBN-13: 9781934115626
- Erscheinungsdatum: 23.10.2008
Sprache:
Englisch
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