Avidin-Biotin Interactions: Methods and Applications
(Sprache: Englisch)
This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert...
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Klappentext zu „Avidin-Biotin Interactions: Methods and Applications “
This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist.
Inhaltsverzeichnis zu „Avidin-Biotin Interactions: Methods and Applications “
- Preparation of avidin conjugates- Coupling of antibodies with biotin
- Optimization of detection and quantification of proteins on membranes in very high and very low abundance using avidin and streptavidin
- Use of the Avidin (Imino)biotin System as a general approach to affinity precipitation
- Capturing biotinylated proteins and peptides by avidin functional affinity electrophoresis
- Functionality Screening of Streptavidin Mutants by Non-Denaturing SDS-PAGE Using Biotin-4-Fluorescein
- Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives
- Quantitative recovery of biotinylated proteins from streptavidin-based affinity chromatography resins
- High yield production and purification of recombinant T7-tag-mature-streptavidin in glucose stressed E. coli
- Endogenous biotin in rat brain
- Pseudo-Immunolabelling with the Avidin-Biotin-Peroxidase Complex (ABC) due to the presence of Endogenous Biotin in the Retina
- Use of synthetic peptides for identifying biotinylation sites in human histones
- Detection and quantitation of DNA methylation using a biotin/avidin microplate assay
- Anti-biotin antibodies offer superior organelle-specific labeling of mitochondria over avidin or streptavidin
- Mammalian lectin as a tool in glycol- and histochemistry with relevance for diagnostic procedure
- A multiplex approach to isotyping antigen-specific antibodies using biotinylated antigen/streptavidin-phycoerythrin
- Biotin-protein bond
Bibliographische Angaben
- 2008, 232 Seiten, Maße: 15,7 x 23,8 cm, Gebunden, Englisch
- Herausgegeben: Robert J. McMahon
- Verlag: Humana Press
- ISBN-10: 1588295834
- ISBN-13: 9781588295835
Sprache:
Englisch
Rezension zu „Avidin-Biotin Interactions: Methods and Applications “
Aus den Rezensionen:"... Das ... editierte Methodenbuch gliedert sich in siebzehn Beiträge, die von auf dem jeweiligen Gebiet ausgewiesenen Autoren verfasst wurden. ... Die angegebenen Protokolle ermöglichen, einem Methodenbuch entsprechend, eine zeitsparende und kostengünstige Präparation ... Positiv ist auch die gute Darstellung der Faktoren, die für Biotinylierungsreaktionen ... kritisch sind. ... Das Buch ist nach Form und Inhalt in jedem Fall geeignet, dem Leser die weitere Vertiefung in die aktuelle Literatur zu ermöglichen und Hinweise für Applikationen zu geben. ... eine umfassende Informationsquelle und Protokolle zur Verfügung ..." (Dr. rer. nat. H. Walzel, in: Laboratoriums-Medizin: journal of laboratory medicine, 2009, Vol.33, Issue 2, S. 129 f.)
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