Bacterial and Archaeal Motility

Purification of the Transmembrane Polypeptide Channel Complex of the Salmonella Flagellar Type III Secretion System.- In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles.- Molecular Simulation to Investigate Open-Close Motion of a Flagellar Export Apparatus Protein FlhAC.- Live-Cell Imaging of the Assembly and Ejection Processes of the Bacterial Flagella by Fluorescence Microscopy.- Purification and CryoEM Image Analysis of the Bacterial Flagellar Filament.- Site-Specific Isotope Labeling of FliG for Studying Structural Dynamics Using Nuclear Magnetic Resonance Spectroscopy.- Site-Directed Crosslinking between Bacterial Flagellar Motor Proteins In Vivo.- Measurements of the Ion Channel Activity of the Transmembrane Stator Complex in the Bacterial Flagellar Motor.- Purification of the Na+-Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopy.- Purification of Na+-Driven MotPS Stator Complexes and Single-Molecule Imaging by High-Speed Atomic Force Microscopy.- High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Having a Rod-Like Straight Hook.- Live-Cell Fluorescence Imaging of Magnetosome Organelle for Magnetotaxis Motility.- Swarming Motility Assays in Salmonella.- Analysis of Adhesion and Surface Motility of a Spirochete Bacterium.- Force Measurement of Bacterial Swimming Using Optical Tweezers.- Archaella Isolation.- Direct Observation of Archaellar Motor Rotation by Single-Molecular Imaging Techniques.- In Situ Structure Determination of Bacterial Surface Nanomachines Using Cryo-Electron Tomography.- Twitching Motility Assays of Lysobacter enzymogenes OH11 Under a Light Microscope.- Live Cell Imaging of the Twitching Motility of Cyanobacteria by High-Resolution Microscopy.- Isolation and Visualization of Gliding Motility Machinery in Bacteroidota.- Live Cell Imaging of Gliding Motility of Flavobacterium johnsoniaeUnder High-Resolution Microscopy.- Social Motility Assays