Bacteriophages
Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions
(Sprache: Englisch)
Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and...
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Klappentext zu „Bacteriophages “
Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 1: Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and molecular characterization, and interaction with bacteria. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.
Inhaltsverzeichnis zu „Bacteriophages “
- Preface - Contributors
- Introduction
Section 1. Bacteriophage Isolation
1. Methods for the Isolation of Viruses from Environmental Samples K. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton, Shellie R. Bench, and Danielle M. Winget
2. Bacteriophage Enrichment from Water and Soil Rohan Van Twest and Andrew M. Kropinski
3. Isolation of Phage via Induction of Lysogens Raúl R. Raya and Elvira M. Hébert
4. Isolation of Cyanophages from Aquatic Environments Andrew D. Millard
5. Isolation of Viruses from High Temperature Environments Jennifer Fulton, Trevor Douglas, and Mark Young
6. Isolation of Novel Large and Aggregating Bacteriophages Philip Serwer, Shirley J. Hayes, Julie Thomas, Borries Demeler, and Stephen C. Hardies
Section 2. Bacteriophage Characterization
7. Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay Andrew M. Kropinski, Amanda Mazzocco, Tom Waddell, Erika Lingohr, and Roger P. Johnson
8. Enumeration of Bacteriophages by the Direct Plating Plaque Assay Amanda Mazzocco, Tom Waddell, Erika Lingohr, and Roger P. Johnson
9. Enumeration of Bacteriophages Using the Small Drop Plaque Assay System Amanda Mazzocco, Tom Waddell, Erika Lingohr, and Roger P. Johnson
10. Determination of Virus Abundance by Epifluorescence Microscopy Alice C. Ortmann and Curtis A. Suttle
11. Enumeration of Bacteriophages Using Flow Cytometry Corina P. D. Brussaard
12. Basic Phage Electron Microscopy Hans-W. Ackermann
13. Phage Classification and Characterization Hans-W. Ackermann
14. Phage Host Range and Efficiency of Plating Elizabeth Kutter
15. Measurement of the Rate of Attachment of Bacteriophage to Cells Andrew M. Kropinski
16. Measurement of the Bacteriophage Inactivation Kinetics with Purified Receptors Andrew M. Kropinski
17. Bacteriophage Plaques: Theory and Analysis Stephen T. Abedon and John Yin
18. Practical Methods for Determining Phage Growth Parameters Paul Hyman
... mehr
and Stephen T. Abedon
19. Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes Louis-Charles Fortier and Sylvain Moineau
Section 3. Bacteriophage-Host Interactions
20. Construction of Phage Mutants (General) Robert Villafane
21. Modifying Bacteriophage l with Recombineering Lynn C. Thomason, Amos B. Oppenheim, and Donald L. Court
22. Identification and Isolation of Lysogens with Induced Prophage Jonathan Livny, Christopher N. LaRock, and David I. Friedman
23. Generalized Transduction Anne Thierauf, Gerardo Perez, and Stanley Maloy
24. Preparation and Characterization of Polyclonal Anti-Phage Serum Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco, and Roger P. Johnson
25. Generalized Transduction by Lytic Bacteriophages Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco, Andrew M. Kropinski, and Roger P. Johnson
19. Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes Louis-Charles Fortier and Sylvain Moineau
Section 3. Bacteriophage-Host Interactions
20. Construction of Phage Mutants (General) Robert Villafane
21. Modifying Bacteriophage l with Recombineering Lynn C. Thomason, Amos B. Oppenheim, and Donald L. Court
22. Identification and Isolation of Lysogens with Induced Prophage Jonathan Livny, Christopher N. LaRock, and David I. Friedman
23. Generalized Transduction Anne Thierauf, Gerardo Perez, and Stanley Maloy
24. Preparation and Characterization of Polyclonal Anti-Phage Serum Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco, and Roger P. Johnson
25. Generalized Transduction by Lytic Bacteriophages Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco, Andrew M. Kropinski, and Roger P. Johnson
... weniger
Bibliographische Angaben
- 2010, Repr. of hardcover 1st ed. 2009, XXII, 308 Seiten, Maße: 19,5 x 26,2 cm, Kartoniert (TB), Englisch
- Herausgegeben: Martha R. J. Clokie, Andrew Kropinski
- Verlag: Springer, Berlin
- ISBN-10: 1617377155
- ISBN-13: 9781617377150
Sprache:
Englisch
Pressezitat
From the reviews: "This book apart from the numerous texts recently published in the area of bacteriophage biology is the focus on the methodology used in this field. ... This volume focuses largely on the classical protocols - as described by the editors ... . This book should prove to be a valuable resource to even the experienced phage biologist and an essential reference book for postgraduate students and scientists new to the field of phage biology." (Sophie Foley, Microbiology Today, July, 2009)
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