Baculovirus and Insect Cell Expression Protocols
(Sprache: Englisch)
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory...
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Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology(TM) series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins. TOC:From the contents
Part I. Introduction.- Part II. Baculovirus Molecular Biology/Development of Recombinant Baculoviruses.- Part III. Insect Cell Culture.- Part IV. Protein Production with Recombinant Baculoviruses.- Part V. Recombinant Protein Production with Transformed Insect Cells.- Part VI. Baculovirus Development and Production for use as Insecticides.- Part VII. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System.
Klappentext zu „Baculovirus and Insect Cell Expression Protocols “
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology(TM) series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.
Inhaltsverzeichnis zu „Baculovirus and Insect Cell Expression Protocols “
- Table of ContentsPART I. INTRODUCTION
- Chapter 1 Useful Tips, Widely Used Techniques and Quantifying Cell Metabolic Behavior
- David W. Murhammer
PART II. BACULOVIRUS MOLECULAR BIOLOGY/DEVELOPMENT OF RECOMBINANT BACULOVIRUSES
- Chapter 2 Introduction to Baculovirus Molecular Biology
- Barbara J. Kelly, Linda A. King, and Robert D. Possee
- Chapter 3 Baculovirus Transfer Vectors
- Robert D. Possee and Linda A. King
- Chapter 4 Recombinant Baculovirus Isolation
- Linda A. King, Richard Hitchman, and Robert D. Possee
- Chapter 5 Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System
- James A. Fornwald, Quinn Lu, Dayuan Wang, and Robert S. Ames
PART III. INSECT CELL CULTURE
- Chapter 6 Available Lepidopteran Insect Cell Lines
- Dwight E. Lynn
- Chapter 7 Lepidopteran Insect Cell Line Isolation From Insect Tissue
- Dwight E. Lynn
- Chapter 8 Development of Serum-Free Media for Lepidopteran Insect Cell Lines
- Sprios N. Agathos
- Chapter 9 Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses
- Dwight E. Lynn
PART IV. PROTEIN PRODUCTION WITH RECOMBINANT BACULOVIRUSES
- Chapter 10 Small Scale Protein Production With the Baculovirus Expression Vector System
- Steve Harwood
- Chapter 11 Recombinant Protein Production in Large Scale Agitated Bioreactors Using the Baculovirus Expression Vector System
- Cynthia B. Elias, Barbara Jardin, and Amine Kamen
- Chapter 12 Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors
- Sue H. Kadwell and Philip I. Hardwicke
- Chapter 13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae
- Yi Liu, Nathan DeCarolis, and Nikolai van Beek
- Chapter 14 Virus-Like Particle and Viral Vector Production Using the BEVS/Insect Cell System: Adeno-Associated Virus Based Products
- Marc G. Aucoin, Danielle Jacob, Parminder S. Chahal, Jamal Meghrous, Alice Bernier, and Amine A.
... mehr
Kamen
PART V. RECOMBINANT PROTEIN PRODUCTION WITH TRANSFORMED INSECT CELLS
- Chapter 15 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression
- Robert L. Harrison and Donald L Jarvis
- Chapter 16 Transformation of Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression
- Lucy Cherbas and Peter Cherbas
- Chapter 17 Transforming Lepidopteran Insect Cells for Improved Protein Processing
- Robert L. Harrison and Donald L Jarvis
PART VI. BACULOVIRUS DEVELOPMENT AND PRODUCTION FOR USE AS INSECTICIDES
- Chapter 18 Introduction to the Use of Baculoviruses as Biological Insecticides
- Bryony C. Bonning and Tyasning Nusawardani
- Chapter 19 Baculovirus Insecticide Production in Insect Larvae
- Nikolai van Beek and David C. Davis
- Chapter 20 Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses
- Huarong Li and Bryony C. Bonning
PART VII. MISCELLANEOUS TECHNIQUES AND APPLICATIONS OF THE BACULOVIRUS/INSECT CELL SYSTEM
- Chapter 21 Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy
- Hyung Joon Cha and William E. Bentley
- Chapter 22 Alternative Molecular and Reactor Strategies Probing Infection and Production
- Yu-Chen Hu and William E. Bentley
- Chapter 23 Methods For Gene Silencing with RNAi
- John C. March and William E. Bentley
- Chapter 24 Use of the Baculovirus/Insect Cell System to Study Apoptosis
- Nor Chejanovsky
- Chapter 25 Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells
- Christian Hofmann
PART V. RECOMBINANT PROTEIN PRODUCTION WITH TRANSFORMED INSECT CELLS
- Chapter 15 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression
- Robert L. Harrison and Donald L Jarvis
- Chapter 16 Transformation of Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression
- Lucy Cherbas and Peter Cherbas
- Chapter 17 Transforming Lepidopteran Insect Cells for Improved Protein Processing
- Robert L. Harrison and Donald L Jarvis
PART VI. BACULOVIRUS DEVELOPMENT AND PRODUCTION FOR USE AS INSECTICIDES
- Chapter 18 Introduction to the Use of Baculoviruses as Biological Insecticides
- Bryony C. Bonning and Tyasning Nusawardani
- Chapter 19 Baculovirus Insecticide Production in Insect Larvae
- Nikolai van Beek and David C. Davis
- Chapter 20 Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses
- Huarong Li and Bryony C. Bonning
PART VII. MISCELLANEOUS TECHNIQUES AND APPLICATIONS OF THE BACULOVIRUS/INSECT CELL SYSTEM
- Chapter 21 Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy
- Hyung Joon Cha and William E. Bentley
- Chapter 22 Alternative Molecular and Reactor Strategies Probing Infection and Production
- Yu-Chen Hu and William E. Bentley
- Chapter 23 Methods For Gene Silencing with RNAi
- John C. March and William E. Bentley
- Chapter 24 Use of the Baculovirus/Insect Cell System to Study Apoptosis
- Nor Chejanovsky
- Chapter 25 Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells
- Christian Hofmann
... weniger
Bibliographische Angaben
- 2007, 2nd ed., XIV, 470 Seiten, Maße: 16,2 x 22,9 cm, Gebunden, Englisch
- Herausgegeben: David W. Murhammer
- Verlag: Springer, Berlin
- ISBN-10: 1588295370
- ISBN-13: 9781588295378
Sprache:
Englisch
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