Basic Techniques in Biochemistry, Microbiology and Molecular Biology

Chapter 1. BOD (Biochemical oxygen demand or biological oxygen demand) Incubator.- Chapter 2. Laminar Air Flow/ Biosafety Cabinets.- Chapter 3. Aseptic Hood.- Chapter 4. Autoclave.- Chapter 5. Hot Air Oven.- Chapter 6. Deep Freezer (-20 0C) (Low Temperature Cabinet).- Chapter 7. Refrigerator.- Chapter 8. Compound Microscope.- Chapter 9. Digital Colony Counter.- Chapter 10. Digital Turbidity Meter.- Chapter 11. Digital Nephel Ometer.- Chapter 12. Digital Colorimeter.- Chapter 13. Digital UV / Visible Spectrophotometer.- Chapter 14. Polymerase Chain Reaction.- Chapter 15. ELISA Reader.- Chapter 16. Sonicator.- Chapter 17. Enzyme Assay: Qualitative and Quantitative (Protease, Amylase, Penicillinase, Pectinolytic Enzyme, Chitinase, Keratinase).- Chapter 18. Antimicrobial Sensitivity Assay.- Chapter 19. Extraction of DNA from plant cells.- Chapter 20. Extraction of DNAfrom Animal cells.- Chapter 21. Extraction of DNA and RNA from microorganism.- Chapter 22. Estimation of DNA by Diphenylamine Reaction.- Chapter 23. Estimation of RNA using Orcinol Method.- Chapter 24. Determination of Melting Temperature of DNA.- Chapter 25. Quantitative Estimation of DNA AND RNA.- Chapter 26. Purification and Bioassay of Interleukin 1 and 2 (IL 1 AND IL 2).- Chapter 27. Object: Quantitative Determination of TNF Alpha and Interleukin 6 in the given sample.- Chapter 28. Sterilization of Glassware, Preparation of Media and its Sterilization.- Chapter 29. Sub Culturing of Bacteria, Fungi and Actinomycetes.- Chapter 30. Preservation of Microorganisms: Stabs, Slants, Lyophilization, Cryopreservation.- Chapter 31. Staining Methods: Simple, Negative, Gram Staining, Acid-Fast Staining, Cell Wall, Capsule, Flagella, Nuclear material, Cytoplasm, Filamentous Fungi, Mycoplasma.- Chapter 32. Isolation of Coprophilous Fungi (By

AAKANCHHA JAIN is an Associate Professor at Bhagyoday Tirth Pharmacy College, Sagar, India. She possesses a throughout first class career. She has to her credit 11 national awards including Nagarjuna Award 2010, Gour Samman 2010, Ranbaxy Science Scholar 2012, and MP Young Scientist Award 2018. She is a Zealous Teacher and Researcher. She has published 23 review and research articles in peer-reviewed high-indexed journals. She was formerly INSPIRE DST Research Fellow at the Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, India, from where she has completed her PhD, MPharm, and BPharm. She has also published two invited book chapters (Wiley, Pan Stanford) covering wide aspects of biotechnology demonstrating her innovation and creativity in delivering lasting results in the field. She has examined many aspects of drug delivery through nanoparticles in her MPharm and PhD. Her main contributions have been in the field of formulation and characterization of novel drug delivery systems, transdermal drug delivery, and anticancer drug delivery systems.

RICHA JAIN is a Senior Scientist, working in the Centre for Scientific Research and Development, People's University, Bhopal, India, possesses more than 14 years of research experience in the area of molecular biology, animal cell culture, microbiology, bioremediation, and industrial and therapeutic enzymes. Presently, she is working on two projects: (a) therapeutic enzymes arginases (anticancer) and (b) bioremediation of plastic waste. She has published 21 research papers with 81 citations in research journals of high repute. Among several feathers on her cap includes Best Paper Award for the presentation of her paper on "Bioinformatics: Importance of Proteomics and Genomics" during the National Conference on "New Frontiers of Sciences" National Science Day Celebrations 2003, Dr. Hari Singh Gour University, Sagar, 28 February 2003; All India Young Scientist Award forthe presentation of her paper