Bioconjugation Protocols

Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities.- Enzymatically-Catalyzed Conjugation of a Biodegradable Polymer to Proteins and Small Molecules Using Microbial Transglutaminase.- Synthesis of Drug/Dye-Incorporated Polymer-Protein Hybrids.- Dye/DNA Conjugates as Multiple Labels for Antibodies in Sensitive Fluorescence Immunoassays.- Chemoselective Modification of Viral Proteins Bearing Metabolically Introduced 'Clickable' Amino Acids and Sugars.- Preparation of Peptide and Other Biomolecular Conjugates through Chemoselective Ligations.- New Fluorescent Substrates of Microbial Transglutaminase and Its Application to Peptide Tag-Directed Covalent Protein Labeling.- Covalent Conjugation of Poly(Ethylene Glycol) to Proteins and Peptides: Strategies And Methods.- Extending the Scope of Site-Specific Cysteine Bioconjugation by Appending a Prelabeled Cysteine Tag to Proteins Using Protein Trans-Splicing.- Polyethylenimine Bioconjugates for Imaging and DNA Delivery In Vivo.- Synthesis of a Glycomimetic Oligonucleotide Conjugate By 1,3-Dipolar Cycloaddition.- Site-Specific DNA Labeling by Staudinger Ligation.- Improved Cellular Uptake of Antisense Peptide Nucleic Acids (PNAs) by Conjugation to a Cell-Penetrating Peptide and a Lipid Domain.- Synthesis of Oligonucleotide-Peptide Conjugates for Biomedical and Technological Applications.- Amphiphilic DNA Block Copolymers: Nucleic Acid-Polymer Hybrid Materials for Diagnostics and Biomedicine.- Chemically Selective Liposome Surface Glyco-Functionalization.- Bioconjugation Using Mutant Glycosyltransferases for the Site-Specific Labeling of Biomolecules with Sugars Carrying Chemical Handles.- Lipid-Core-Peptide Systemfor Self-Adjuvanting Synthetic Vaccine Delivery.- Coupling Carbohydrates to Proteins for Glycoconjugate Vaccine Development Using a Pentenoyl Group as a Convenient Linker.- Conjugation of LPS-Derived Oligosaccharides to Proteins Using Oxime