Micro and Nano Technologies in Bioanalysis
Methods and Protocols
(Sprache: Englisch)
In recent years, large-scale advances in technology have led to greater understanding of the world at the biomolecular level. In this book, expert researchers from across the globe explore the technology which makes this analysis possible.
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In recent years, large-scale advances in technology have led to greater understanding of the world at the biomolecular level. In this book, expert researchers from across the globe explore the technology which makes this analysis possible.
Klappentext zu „Micro and Nano Technologies in Bioanalysis “
In recent years, large-scale advances in technology have led to greater understanding of the world on a much tinier scale: the biomolecular level. In Micro and Nano Technologies in Bioanalysis: Methods and Protocols, expert researchers from across the globe explore the technology which makes this analysis possible, investigating the worlds of microfluidics and nanotechnologies, and examining physical science techniques for the separation, detection, manipulation, and analysis of biomolecules. This volume contains innovative protocols on the application of microfluidics and the utilization of physical science-related technologies that will prove to be invaluable in the field of molecular biology. Chapters contain cutting edge applications of emerging nanotechnologies, including quantum dots and molecular fluorescence for the imaging and tracking of biomolecules. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and groundbreaking, Micro and Nano Technologies in Bioanalysis: Methods and Protocols is a necessary tool for cellular biologists, biochemists, microbiologists, geneticists and medical researchers alike.
Inhaltsverzeichnis zu „Micro and Nano Technologies in Bioanalysis “
Table of ContentsPreface
Contributors
Part I. Applications of microfluidics and nanopores in separation, detection, manipulation, and analysis of biomolecules
1. HPLC-Chip/MS technology in proteomic profiling
Martin Vollmer and Tom van de Goor
2. Nanofluidic channels fabrication and manipulation of DNA molecules
Kai-Ge Wang, Hanben Niu
3. A single-molecule barcoding system using nanoslits for DNA analysis--Nanocoding
Kyubong Jo, Timothy M. Schramm, David C. Schwartz
4. Microfluidic Devices with Photodefinable Pseudo-valves for Protein Separation
Z. Hugh Fan
5. Microfluidic chip designed for measuring biomolecules through microbead-based quantum dot fluorescence assay
Kwang-Seok Yun, Dohoon Lee, Hak-Sung Kim and Euisik Yoon
6. DNA Focusing Using Microfabricated Electrode Arrays
Faisal A. Shaikh and Victor M. Ugaz
7. Solid-state Nanopore for Detecting Individual Biopolymers
Jiali Li and Jene A. Golovchenko
8. Inserting and manipulating DNA in a nanopore with optical tweezers
U. F. Keyser, J. van der Does, C. Dekker, N. H. Dekker
9. Forming an a-hemolysin nanopore for single molecule analysis
Nahid N. Jetha, Matthew Wiggin, and Andre Marziali
10. Nanopore Force Spectroscopy on DNA Duplexes
Nahid N. Jetha, Matthew Wiggin, and Andre Marziali
Part II. Technologies of physical science and chemistry in detection and analysis of biomolecules
11. Quantitative chemical analysis of single cells
Michael L. Heien and Andrew G. Ewing
12. Trapping and Detection of Single Molecules in Water
M. Willander, K. Risveden, B. Danielsson and O. Nur
13. ZnO Nanorods as an Intracellular Sensor for pH Measurements
M. Willander and Safaa Al-Hilli
14. Analysis of Biomolecules Using Surface Plasmons
M. Willander and Safaa Al-Hilli
15. Use of residual dipolar couplings in structural analysis of protein-ligand complexes by solution NMR spectroscopy
Nitin U. Jain
16. Raman-assisted X-ray crystallography for the analysis of
... mehr
biomolecules
Dominique Bourgeois, Gergely Katona, Eve de Rosny and Philippe Carpentier
17. Methods and Software for Diffuse X-Ray Scattering from Protein Crystals
Michael E. Wall
18. Deuterium Labeling for Neutron Structure-Function-Dynamics Analysis
Flora Meilleur, Kevin L. Weiss, and Dean A.A. Myles
19. Small-angle Neutron Scattering for Molecular Biology: Basics and Instrumentation
William T. Heller, Kenneth C. Littrell
20. Small-Angle Scattering and Neutron Contrast Variation for Studying Bio-molecular Complexes
Andrew E. Whitten and Jill Trewhella
21. Protein sequencing with tandem mass spectrometry
Assem G. Ziady and Michael Kinter
22. Metabolic Analysis
Vladimir V. Tolstikov
Part III. Applications of quantum dots and molecular fluorescence in detection, tracking and imaging of biomolecules
23. Multicolor detection of combed DNA molecules using quantum-dots
Christophe Escudé, Bénédicte Géron-Landre, Aurélien Crut, and Pierre Desbiolles
24. Quantum Dot Molecular Beacons for DNA Detection
Nathaniel C. Cady
25. Quantum dot hybrid gel blotting: a technique for identifying quantum dot-protein/ protein-protein interactions
Tania Q. Vu and Hong Yan Liu
26. In vivo imaging of quantum dots
Isabelle Texier, Véronique Josserand
27. Semiconductor Fluorescent Quantum dots: Efficient biolabels in cancer diagnostics
Patricia M. A. Farias, Beate S. Santos, Adriana Fontes
28. The Monitoring and Affinity
Dominique Bourgeois, Gergely Katona, Eve de Rosny and Philippe Carpentier
17. Methods and Software for Diffuse X-Ray Scattering from Protein Crystals
Michael E. Wall
18. Deuterium Labeling for Neutron Structure-Function-Dynamics Analysis
Flora Meilleur, Kevin L. Weiss, and Dean A.A. Myles
19. Small-angle Neutron Scattering for Molecular Biology: Basics and Instrumentation
William T. Heller, Kenneth C. Littrell
20. Small-Angle Scattering and Neutron Contrast Variation for Studying Bio-molecular Complexes
Andrew E. Whitten and Jill Trewhella
21. Protein sequencing with tandem mass spectrometry
Assem G. Ziady and Michael Kinter
22. Metabolic Analysis
Vladimir V. Tolstikov
Part III. Applications of quantum dots and molecular fluorescence in detection, tracking and imaging of biomolecules
23. Multicolor detection of combed DNA molecules using quantum-dots
Christophe Escudé, Bénédicte Géron-Landre, Aurélien Crut, and Pierre Desbiolles
24. Quantum Dot Molecular Beacons for DNA Detection
Nathaniel C. Cady
25. Quantum dot hybrid gel blotting: a technique for identifying quantum dot-protein/ protein-protein interactions
Tania Q. Vu and Hong Yan Liu
26. In vivo imaging of quantum dots
Isabelle Texier, Véronique Josserand
27. Semiconductor Fluorescent Quantum dots: Efficient biolabels in cancer diagnostics
Patricia M. A. Farias, Beate S. Santos, Adriana Fontes
28. The Monitoring and Affinity
... weniger
Bibliographische Angaben
- 2009, 688 Seiten, Maße: 19,8 x 26,6 cm, Gebunden, Englisch
- Herausgegeben: Robert S. Foote, James W. Lee
- Verlag: Humana Press
- ISBN-10: 1934115401
- ISBN-13: 9781934115404
- Erscheinungsdatum: 21.07.2009
Sprache:
Englisch
Rezension zu „Micro and Nano Technologies in Bioanalysis “
From the reviews:"This book provides a comprehensive overview of different modern methods with acknowledged practical interest. ... this book can be considered as unique and highly valuable. ... This book can be recommended for scientists, technicians and students in the fields of molecular and cell biology, biochemistry, microbiology, genetics, medical research and analytical chemistry. ... This book is an excellent source for interested users to learn about recent developments of emerging microtechnologies and nanotechnologies for bioanalysis." (Rosario Pereiro, Analytical and Bioanalytical Chemistry, November, 2009)"This book describes specific micro and nano techniques used in analyzing functions in both individual cells and animal models. It includes details and tips for using these methods. ... Written for scientists at many different levels, the book includes specific step-by-step instructions in each chapter that would be useful for senior scientists as well as bench technical assistants. ... will be a valuable resource for biomedical scientists." (Rebecca T. Horvat, Doody's Review Service, February, 2010)
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