Brassinosteroids

Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues.- Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye.- Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice.- Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness.- Approaches to Study Light Effects on Brassinosteroid Sensitivity.- A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity.- Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing.- Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Associated Polyadenylated RNA.- Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development.- Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells.- Analysis of In Vitro DNA Interactions of Brassinosteroid-Controlled Transcription Factors Using Electrophoretic Mobility Shift Assay.- Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometry.- Simplified Enrichment of Plasma Membrane Proteins from Arabidopsis thaliana Seedlings Using Differential Centrifugation and Brij-58 Treatment.- Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation.- The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops.- Brassinosteroid Action in Plant Abiotic Stress Tolerance.