Cellular Bioenergetics: Role of Coupled Creatine Kinases

Preface.- I - Muscle Energy Metabolism.- 1a. Introduction: history of the problem.- 1b. A brief summary of the history of the detection of creatine kinase isoenzymes.- 2. Metabolic regulation of in vivo myocardial contractile function: multiparameter analysis.- 3. Compartmentation of ATP synthesis and utilization in smooth muscle: roles of aerobic glycolysis and creatine kinase.- 4. Creatine metabolism and the consequences of creatine depletion in muscle.- II - Substrate and Creatine Kinase Isoenzyme Compartmentation.- 1. The importance of the outer mitochondrial compartment in regulation of energy metabolism.- 2. The influence of the cytosoliconcotic pressure on the permeability of the mitochondrial outer membrane for ADP: implications for the kinetic properties of mitochondrial creatine kinase and for ADP channelling into the intermembrane space.- 3. Influence of mitochondrial creatine kinase on the mitochondrial/extramitochondrial distribution of high energy phosphates in muscle tissue: evidence for a leak in the creatine shuttle.- 4. The structure of mitochondrial creatine kinase and its membrane binding properties.- 5. Myofibrillar creatine kinase and cardiac contraction.- 6. Interaction of creatine kinase and adenylate systems in muscle cells.- III - Creatine Kinases and Metabolic Integration.- 1. Metabolic compartmentation and substrate channelling in muscle cells. Role of coupled creatine kinases in in vivo regulation of cellular respiration - a synthesis.- 2. Creatine kinase in non-muscle tissues and cells.- 3. The creatine kinase system in smooth muscle.- IV - Molecular Biology of Creatine Kinases.- 1. Expression of the mitochondrial creatine kinase genes.- 2. Sequence homology and structure predictions of the creatine kinase isoenzymes.- 3.Approaching the multifaceted nature of energy metabolism: inactivation of the cytosolic creatine kinases via homologous recombination in mouse embryonic stem cells.- V - Development and Pathological Alterations of