Crop Breeding
Methods and Protocols
(Sprache: Englisch)
The recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level. Crop Breeding: Methods and Protocols presents detailed guidelines and tutorials...
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The recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level. Crop Breeding: Methods and Protocols presents detailed guidelines and tutorials that suit different needs and capacity from small laboratories analyzing molecular markers on a one-by-one basis to the increasingly popular high-throughput protocols for high capacity laboratories. Topics covered include breeding strategy for the selection of an ideal variety or genetic ideo type, protocols for breeders using molecular markers in selection programs and for laboratories providing molecular services to breeding programs, statistical programs and software to aid implementation of molecular data into breeding programs and methodologies that facilitate the generation of genetic diversity and its characterization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Crop Breeding: Methods and Protocols will help in expanding the use of molecular technologies for the creation of tomorrow's crop varieties.
Inhaltsverzeichnis zu „Crop Breeding “
1. Defining a Genetic Ideotype for Crop ImprovementRichard M. Trethowan
2. From Genes to Markers: Exploiting Gene Sequence Information to Develop Tools for Plant Breeding
Melissa Garcia and Diane E. Mather
3. Temperature Switch PCR (TSP) - A Gel-Based Molecular Marker Technique for Investigating Single Nucleotide Polymorphisms
Thanh Le Phuoc and Kelvin Khoo
4. Multiplex-Ready Technology for Mid-Throughput Genotyping of Molecular Markers
Julien Bonneau and Matthew Hayden
5. Genotyping by High-Resolution Melting Analysis
Elise J. Tucker and Bao Lam Huynh
6. Bi-Allelic SNP Genotyping Using the TaqMan® Assay
John Woodward
7. SNP Genotyping: The KASPTM Assay
Chunlin He, John Holme, and Jeffrey Anthony
8. Rindsel: An R Package for Phenotypic and Molecular Selection Indices Used in Plant Breeding
S. Perez-Elizalde, J. J. Ceron-Rojas, J. Crossa, Delphine Fleury, and G. Alvarado
9. OptiMAS: A Decision Support Tool to Conduct Marker-Assisted Selection Programs
Fabio Valente, Franck Gauthier, Nicolas Bardol, Guylaine Blanc, Johann Joets, Alain Charcosset, and Laurence Moreau
10. Genomic Selection in Plant Breeding
M. A. Newell and J.-L Jannink
11. Simulated Breeding with QU-GENE Graphical User Interface
Adrian Hathorn, Sctoo Chapman, and Mark Dieters
12. The Control of Recombination in Wheat by Ph1 and Its Use in Breeding
Graham Moore
13. TILLING for Plant Breeding
Peter Sharp and Chongmei Dong
14. In vitro Culture for Doubled Haploids: Tools for Molecular Breeding
Sue Broughton, Parminder K. Sidhu, and Philip A. Davies
15. Biolistic Transformation of Wheat with Centrophenoxine as a Synthetic Auxin
Ainur Ismagul, Gulnur Oskakova, John C. Harris, and Serik Eliby
16. Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.)
Ainur Ismagul, Iryna Mazonka, Corrine Callegari, and Serik Eliby
17. qPCR for Quantification of Transgene Expression and Determination of Transgene
... mehr
Copy Number
Stephen J. Fletcher
18. High-Throughput Analysis Pipeline for Achieving Simple Low-Copy Wheat and Barley Transgenics
Nataliya Kovalchuk
Stephen J. Fletcher
18. High-Throughput Analysis Pipeline for Achieving Simple Low-Copy Wheat and Barley Transgenics
Nataliya Kovalchuk
... weniger
Bibliographische Angaben
- 2014, 2014, X, 255 Seiten, 58 farbige Abbildungen, Maße: 18,3 x 25,8 cm, Gebunden, Englisch
- Herausgegeben: Delphine Fleury, Ryan Whitford
- Verlag: Springer, Berlin
- ISBN-10: 1493904450
- ISBN-13: 9781493904457
Sprache:
Englisch
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