cryoEM

Getting Started with in situ cryo-electron Tomography.- Cryo-Electron Tomography and Automatic Segmentation of Cultured Hippocampal Neurons.- Practical Approaches for Cryo-FIB Milling and applications for Cellular Cryo-Electron Tomography.- In situ Imaging and Structure Determination of Biomolecular Complexes using Cryo-Electron Tomography.- Progress Towards CryoEM: Negative-stain Procedures for Biological Samples.- Setting up Parallel Illumination on the Talos Arctica for High-Resolution Data Collection.- Multi-body refinement of cryo-EM images in RELION.- Evaluating Local and Directional Resolution of Cryo-EM Density Maps.- Automated modeling and Validation of Protein Complexes in cryo-EM Maps.- 2D Electron Crystallography of Membrane Protein Single-, Double- and Multi-Layered Ordered Arrays.- Sample Preparation and Data Collection for Electron Crystallographic Studies on Membrane Protein Structures and Lipid-ProteinInteraction.- Single Particle Analysis for High Resolution 2D Electron Crystallography.- MicroED Sample Preparation and Data Collection for Protein Crystals.-- Detection of Microcrystals for CryoEM.- Low-dose Data Collection and Radiation Damage in MicroED.- Automation of Continuous-rotation Data Collection for MicroED.- Ab initio Determination of Peptide Structures by MicroED.