Delivering the Message: Cytoplasmic mRNA Localization In Yeast; .
Biochemical Purification and Functional Characterization of the She RNP Complex
(Sprache: Englisch)
Asymmetric mRNA localization is a widely used mechanism to sort cell fate determinants in development. In budding yeast ASH1 mRNA localization to the growing bud leads to targeting of Ash1p to the daughter cell, a prerequisite for proper mating type...
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Asymmetric mRNA localization is a widely used mechanism to sort cell fate determinants in development. In budding yeast ASH1 mRNA localization to the growing bud leads to targeting of Ash1p to the daughter cell, a prerequisite for proper mating type switching. In a genetic screen 5 SHE genes required for ASH1 mRNA localization have been isolated. SHE1 is equivalent to MYO4 - a locus that encodes a myosin - suggesting a cytoskeleton-based, active transport mechanism. Functional characterization in turn has implied Myo4p as the motor protein, She2p as the ASH1 mRNA-binding protein, and She3p as adapter protein connecting She2p to Myo4p. Accessory factors such as Loc1p or Khd1p have been reported to function in ASH1 mRNA localization though they have not been identified in the genetic screen. To allow a detailed characterization of the ASH1-She RNP (ribo-nucleoprotein) complex, I initiated a tandem affinity purification protocol, using either She2p or Myo4p as bait protein. In either case I could purify the core She machinery together with ASH1 mRNA, arguing for the integrity of a single RNP. Furthermore, mass-spectrometric analysis identified a number of so far unknown proteins.
Autoren-Porträt von Andreas Jaedicke
Jaedicke, AndreasThe author has studied cell biology, molecular biology and biochemistry in Mainz, Heidelberg and Munich. Having obtained a Ph. D. in biology in 2004 he has joined the European Molecular Biology Laboratory in Heidelberg.
Bibliographische Angaben
- Autor: Andreas Jaedicke
- 2008, 132 Seiten, Maße: 15 x 22 cm, Kartoniert (TB), Englisch
- Verlag: VDM Verlag Dr. Müller e.K.
- ISBN-10: 3639023137
- ISBN-13: 9783639023138
Sprache:
Englisch
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