Developmental Biology of the Sea Urchin and Other Marine Invertebrates

Culturing Larvae of Marine Invertebrates.- Obtaining Gametes and Embryos of Ascidians.- Artificial Seawater Culture of the Gastropod Crepidula fornicata for Studies of Larval Settlement and Metamorphosis.- Trichoplax adhaerens, an Enigmatic Basal Metazoan with Potential.- Rearing Gymnolaemate Bryozoan Larvae for Cellular and Molecular Analyses.- An Invertebrate Embryologist's Guide to Routine Processing of Confocal Images.- TEM Analyses of Chaetognath Reproductive Organs.- SEM Analysis of Marine Invertebrate Gametes.- Imaging Neural Development in Embryonic and Larval Sea Urchins.- Preparation and Use of Sea Urchin Egg Homogenates.- Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian Phallusia mammillata.- Isolating Specific Embryonic Cells of the Sea Urchin by FACS.- Biotinylation of Oocyte Cell Surface Proteins of the Starfish Patiria miniata.- Perturbations to the Hedgehog Pathway in Sea Urchin Embryos.- Regulation of DNA Synthesis at the First Cell Cycle in the Sea Urchin in vivo.- Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte .- Maturation in Marine Nemertean Worms.- Multi-color Labeling in Developmental Gene Regulatory Network Analysis.- A 96-well Plate Format for Detection of Marine Zooplankton with the Sandwich Hybridization Assay.- Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and Through the Egg-to-Embryo Transition in Sea Urchines.- Tools for Sea Urchin Genomic Analysis.- Antibody Inhibition of Protein Activity in Starfish Oocytes.- Immunophotoaffinity Labeling of the Binding Proteins for 1-Methyladenine, an Oocyte-maturation Inducing Hormone of Starfish.