Dynein

Live-cell imaging of dynein-mediated cargo transport in Aspergillus nidulans.- Using budding yeast as a model to understand dynein-mediated cargo transport.- Employing live-cell imaging to study motor-mediated transport.- Assessment of dynein-mediated nuclear migration in the developing cortex by live-tissue microscopy.- Using optogenetics to spatially control cortical dynein activity in mitotic human cells.- Visualization of single dynein molecules in mammalian cells.- Single-molecule studies of motor adaptors using cell lysates.- Reconstitution of organelle transport along microtubules in vitro.- Reconstitution of dynein/dynactin transport using recombinant dynein.- Fast and easy transient mammalian cell expression and purification of cytoplasmic dynein.- High-resolution tracking of dynein-dynactin-BicD2 complexes.- In vivo trapping of latex bead phagosomes for quantitative force measurements.- Studying dynein mechanochemistry with an optical trap.- Measurements of theForce-Dependent Detachment Rates of Cytoplasmic Dynein from Microtubules.- NMR analysis of the interactions and conformational plasticity of dynein intermediate chain.- High-resolution Structural Analysis of Dyneins by Cryo-electron Microscopy.