Electrophoretic Separation of Proteins

How It All Began: A Personal History of Gel-Electrophoresis.- Introduction to Protein Electrophoresis.- Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilisation, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic Evaluation.- Lysis Buffer Choices are Key Considerations to Ensure Effective Sample Solubilization for Protein Electrophoresis.- The Cydex Blue Assay: A One Step Protein Assay for Samples Prior to SDS Electrophoresis.- Cellulose-Acetate Electrophoresis of Hemoglobin.- Native Polyacrylamide Gels.- Isoelectric Focusing on Non-Denaturing Gels.- Determination of protein Molecular weights on SDS-PAGE.- Two-Dimensional Gel Electrophoresis by Glass Tube-Based IEF and SDS-PAGE.- Cationic Electrophoresis.- Two-Dimensional Gel Electrophoresis with Immobilized pH Gradients.- SARCOSYL-PAGE: Optimized Protocols for the Separation and Immunological Detection of PEGylated Proteins.- Tricine-SDS-PAGE.- Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis.- Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies.- Electrophoretic Separation of Very Large Molecular Weight Proteins in SDS-Agarose.- Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis.- Two-Dimensional Difference Gel Electrophoresis.- Immunoelectrophoresis: A Method with Many Faces.- Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins if Very High and Low Molecular Mass.- Diagnoal Electrophoresis for Detection of Proteins Involved in Disulfide Bonds.- Identification of Proteins on Archived 2D Gels.- Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing.- A