Engineered Zinc Finger Proteins

Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications. TOC:The Generation of Zinc Finger Proteins by Modular Assembly.- Engineering Single Cys2His2 Zinc Finger Domains using a Bacterial Cell-Based Two-Hybrid Selection System.- Bipartite Selection of Zinc Fingers by Phage Display for Any 9-bp DNA Target Site.- Structure-Based DNA Binding Prediction and Design.- Generation of Cell-Permeable Artificial Zinc Finger Protein Variants.- Inhibition of Viral Transcription Using Designed Zinc Finger Proteins.- Modulation of Gene Expression Using Zinc Finger Based Artificial Transcription Factors.- Construction of Combinatorial Libraries that Encode Zinc Finger-Based Transcription Factors.- Silencing of Gene Expression by Targeted DNA Methylation: