Expressed Protein Ligation

 Methods and Applications of Expressed Protein Ligation.- Protein Splicing: From the Foundations to the Development of Biotechnological Applications.- Identification, Characterization, and Optimization of Split Inteins.- Methods to Study the Structure and Catalytic Activity of cis-splicing Inteins.- Expressed Protein Ligation: General Experimental Protocols.- Peptide Hydrazides as Thioester Equivalents for the Chemical Synthesis of Proteins.- Native Chemical Ligation Via N-Acylurea Thioester Surrogates Obtained by Fmoc Solid Phase Peptide Synthesis.-Fused Split Inteins: Tools for Introducing Multiple Protein Modifications.- Streamlined Expressed Protein Ligation: Site-specific Antibody-drug Conjugate.- In vivo Histone Labeling Using Ultrafast trans-Splicing Inteins.- Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation.- Site-specific Phosphorylation of PDZ Domains.- Semisynthesis and Reconstitution of Nucleosomes Carrying Asymmetric Histone Modifications.- Facile Semisynthesis of Ubiquitylated Peptides with the Ligation Auxiliary 2-Aminooxyethanethiol.- Investigating the Effects of O-GlcNAc Modifications in Parkinson's Disease using Semi-synthetic -synuclein.- Recombinant Expression of Cyclotides Using Expressed Protein Ligation.- Highly Precise Protein Semisynthesis through Ligation-Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines.