Fluorescent Methods to Study Biological Membranes

Laurdan Fluorescence Properties in Membranes : A Journey from the Fluorimeter to the Microscope.- Application of NBD-labeled Lipids in Membrane and Cell Biology.- 3-Hydroxychromone Probes Precisely Located and Oriented in Lipid Bilayers: a Toolkit for Biomembrane Research.- Lateral Membrane Heterogeneity Probed by FRET Spectroscopy and Microscopy.- FRET Analysis of Protein-Lipid Interactions.- Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shifts.- Visual Discrimination of Membrane Domains in Live Cells by Wide-Field Microscopy.- Quantitative Fluorescence Studies of Intracellular Sterol Transport and Distribution.- Studying Membrane Properties Using Fluorescence Lifetime Imaging Microscopy (FLIM).- Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions.- Deciphering Cell Membrane Organization Based on Lateral Diffusion Measurements by Fluorescence Correlation Spectroscopy at Different Length Scales.- STED-FCS Nanoscopy of Membrane Dynamicsl.- Imaging Molecular Order in Cell Membranes by Polarization Resolved Fluorescence Microscopy.- Near-Field Optical Nanoscopy of Biological Membranes.- Unveiling Biophysical and Biological Properties of a Hypothetical Membrane Receptor by Exploiting Recent Imaging Advances.- New Fluorescent Strategies Shine Light on the Evolving Concept of GCPR Oligomerization.- Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dymamics of the Serotonin1A Receptor.- TNF Receptor Membrane Dynamics Studied with Fluorescence Microscopy and Spectroscopy.- HIV-1 Gag Directed Assembly of Retroviral Particles Investigated by Quantitative Fluorescence Imaging.

From the reviews:"The book covers all major applications of fluorescence to membrane studies. ... The book is addressed to scientists and PhD students using fluorescence methods to study biological membranes and membrane proteins. This is a highly interdisciplinary field, and the book will therefore be of interest to biophysicists, physical chemists, and cellular biologists ... . broad overview of different techniques provided by the book is also a good starting point for newcomers to the field. ... a valuable resource for everyone studying membranes and membrane proteins." (Lorenzo Stella, Analytical and Bioanalytical Chemistry, May, 2013)