Gap Junction Protocols

Analysis of Liver Connexin ExpressionUsing Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.- DNA Methylation Analysis of HumanTissue-Specific Connexin Genes.- Detection of Connexins in Liver Cells UsingSodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.- Immunohisto- andCytochemistry Analysis of Connexins.- Small Interfering RNA-MediatedConnexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.- Generation and Use of TrophoblastStem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancyand Labor.- Identification of Connexin43Phosphorylation and S-Nitrosylationin Cultured Primary Vascular Cells.- Preparation of Gap Junctions inMembrane Microdomains for Immunoprecipitation and Mass Spectrometry InteractomeAnalysis.- Scrape Loading/Dye Transfer Assay.- Microinjection Technique for Assessment of GapJunction Function.- Electroporation Loading and DyeTransfer: A Safe and Robust Method to Probe Gap Junctional Coupling.- Using Fluorescence Recovery After Photobleaching to Study Gap JunctionalCommunication In Vitro.- Tracking Dynamic Gap Junctional Coupling in Live Cells by LocalPhoto-Activation and Fluorescence Imaging.- A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication ofSecond Messengers in Cultured Bone Cells.- Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Methodfor Studying Gap Junctions and Hemichannels.- Establishment of the Dual Whole Cell Recording Patch Clamp Configuration forthe Measurement of Gap Junction Conductance.