Gene Correction

RecTEPsy Mediated Recombineering in Pseudomonas syringae.- Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophila.- Multiple Genetic Manipulations of DT40 Cell Line.- Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombination.- Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cells.- Small Fragment Homologous Replacement (SFHR): Sequence-Specific Modification of Fenomic DNA in Eukaryotic Cells by Small DNA Fragments.- Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotide Donors for Gene Correction.- Triplex-Mediated Genome Targeting and Editing.- Targeting piggyBac Transposon Integrations in the Human Genome.- Gene Targeting in Human Induced Pluripotent Stem Cells with Adenoviral Vectors.- Enhanced Gene Targeting of Adult and Pluripotent Stem Cells Using Evolved Adeno-Associated Virus.- Lentiviral Vectors Encoding Zinc-Finger Nucleases Specific for the Model Target Locus HPRT1.- Designing and Testing the Activities of TAL Effector Nucleases.- A Bacterial One-Hybrid System to Isolate Homing Endonuclease Variants with Altered DNA Target Specificities.- Design and Analysis of Site-Specific Single-Strand Nicking Endonucleases for Gene Correction.- CRISPR-Cas Mediated Targeted Genome Editing in Human Cells.- RNA-Guided Genome Editing of Mammalian Cells.- Nuclease-Mediated Double Strand Break (DSB) Enhancement of Small Fragment Homologous Recombination (SFHR) Gene Modification in Human Induced Pluripotent Stem Cells (hiPSCs).- AAV-Mediated Gene Editing via Double-Strand Break Repair.- Genetic Modification Stimulated by the Induction of a Site-Specific Break Distant from the Locus of Correction in Haploid and Diploid Yeast Saccharomyces cerevisiae.- A Southern Blot Protocol to Detect Chimeric Nuclease-Mediated Gene Repair.- High-Throughput Cellular Screening of Engineered Nuclease Activity Using the Single-Strand Annealing Assay and