Gene Therapy Protocols

Table of Contents 1 Purification of Retrovirus Particles Using Heparin Affinity Chromatography María de las Mercedes Segura, Amine Kamen and Alain Garnier 2 Scaleable Purification of Adenovirus Vectors John O. Konz, Lee R. Pitts and Sangeetha L. Sagar 3 Quantifying the Titer and Quality of Adenovirus Stocks Fubao Wang, Bill C. Mathis, Allison Montalvo, Jayanthi J. Wolf and John A. Lewis 4 Chromatography-Based Purification of Adeno-Associated Virus Richard H. Smith, Linda Yang and Robert M. Kotin 5 Spectroscopic Methods for the Physical Characterization and Formulation of Nonviral Gene Delivery System Salvador F. Ausar, Sangeeta B. Joshi and C. Russell Middaugh 6 Real Time Multiple Particle Tracking of Gene Nanocarriers in Complex Biological Environments Samuel K. Lai and Justin Hanes 7 Production of Lentiviruses displaying 'Early-Acting' Cytokines for Selective Gene Transfer into Hematopoietic Stem Cells E. Verhoeyen, D. Nègre and F.L. Cossett 8 Fiber-modified Adenoviruses for Targeted Gene Therapy Hongju Wu and David T. Curiel 9 PEGylated Adenovirus for Targeted Gene Therapy Catherine R. O'Riordan and Antonius Song 10 Transposon-Based Mutagenesis Generates Diverse Adeno-Associated Viral Libraries with Novel Gene Delivery Properties James T. Koerber and David V. Schaffer 11 Photochemical Enhancement of DNA Delivery by EGF Receptor Targeted Polyplexes Anette Bonsted, Ernst Wagner, Lina Prasmickaite, Anders Høgset and Kristian Berg 12 Reducing the Genotoxic Potential of Retroviral Vectors Ali Ramezani, Teresa S. Hawley and Robert G. Hawley 13 Evaluation of Promoters for Use in Tissue-Specific GeneDelivery Changyu Zheng and Bruce J. Baum 14 Adenovirus-Mediated Transduction of Auto- and Dual-Regulated Transgene Expression In Mammalian Cells Valeria Gonzalez-Nicolini and Martin Fussenegger 15 Regulated expression of Adenoviral Vectors-based Gene Therapies: Therapeutic Expression of Toxins and Immune-modulators James F. Curtin, Marianela Candolfi, Mariana Puntel, Weidong

From the reviews of the third edition: "This book reflects progress in the field with chapters on early stage vector development and in vitro evaluation, as well as others devoted to issues faced in preclinical and clinical development . Readers of this book are likely to be those working in vector development, validation and production as well as those involved in gene-based clinical trials. The specialized nature of this book means that it will primarily be used by experienced researchers ." (Christopher Ring, Microbiology Today, June, 2009)