Genotoxicity and DNA Repair
A Practical Approach
(Sprache: Englisch)
Genotoxicity and DNA Repair: A Practical Approach provides a key reference for determining how to analyze the genotoxic activity of molecules or materials and, at the same time, serves as a useful tool for researchers in the Environmental Mutagenesis and...
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Genotoxicity and DNA Repair: A Practical Approach provides a key reference for determining how to analyze the genotoxic activity of molecules or materials and, at the same time, serves as a useful tool for researchers in the Environmental Mutagenesis and DNA Repair fields. Focused on genotoxicity assays recommended by the "OECD guidelines for the testing of chemicals", this volume also covers other useful assays, such as some gene mutation assays, the comet assay in different species and applications, and the SMART assays of Drosophila. For all the assays, the book presents brief theoretical introductions to the topics and updated standard and modified step-by-step protocols to perform them. Special emphasis is placed on the analysis of nanoparticles, including an integrative approach analysis. The DNA Repair section includes several assays that provide information on repair activity in vitro and in vivo, as well as recent applications to study DNA repair in humans, cell cultures, and animal models. As a volume in the Methods in Pharmacology and Toxicology series, the chapters contain the kind of detail and key implementation advice that ensures reproducible results in the lab.
Authoritative and invaluable, Genotoxicity and DNA Repair: A Practical Approach aims to aid scientists in their pursuit of forwarding this vital field of study.
Authoritative and invaluable, Genotoxicity and DNA Repair: A Practical Approach aims to aid scientists in their pursuit of forwarding this vital field of study.
Klappentext zu „Genotoxicity and DNA Repair “
Genotoxicity and DNA Repair: A Practical Approach provides a key reference for determining how to analyze the genotoxic activity of molecules or materials and, at the same time, serves as a useful tool for researchers in the Environmental Mutagenesis and DNA Repair fields. Focused on genotoxicity assays recommended by the "OECD guidelines for the testing of chemicals", this volume also covers other useful assays, such as some gene mutation assays, the comet assay in different species and applications, and the SMART assays of Drosophila. For all the assays, the book presents brief theoretical introductions to the topics and updated standard and modified step-by-step protocols to perform them. Special emphasis is placed on the analysis of nanoparticles, including an integrative approach analysis. The DNA Repair section includes several assays that provide information on repair activity in vitro and in vivo, as well as recent applications to study DNA repair in humans, cell cultures, and animal models. As a volume in the Methods in Pharmacology and Toxicology series, the chapters contain the kind of detail and key implementation advice that ensures reproducible results in the lab.Authoritative and invaluable, Genotoxicity and DNA Repair: A Practical Approach aims to aid scientists in their pursuit of forwarding this vital field of study.
Genotoxicity and DNA Repair: A Practical Approach provides a key reference for determining how to analyze the genotoxic activity of molecules or materials and, at the same time, serves as a useful tool for researchers in the Environmental Mutagenesis and DNA Repair fields. Focused on genotoxicity assays recommended by the "OECD guidelines for the testing of chemicals", this volume also covers other useful assays, such as some gene mutation assays, the comet assay in different species and applications, and the SMART assays of Drosophila. For all the assays, the book presents brief theoretical introductions to the topics and updated standard and modified step-by-step protocols to perform them. Special emphasis is placed on the analysis of nanoparticles, including an integrative approach analysis. The DNA Repair section includes several assays that provide information on repair activity in vitro and in vivo, as well as recent applications to study DNA repair in humans, cell cultures, and animal models. As a volume in the Methods in Pharmacology and Toxicology series, the chapters contain the kind of detail and key implementation advice that ensures reproducible results in the lab.
Authoritative and invaluable, Genotoxicity and DNA Repair: A Practical Approach aims to aid scientists in their pursuit of forwarding this vital field of study.
Authoritative and invaluable, Genotoxicity and DNA Repair: A Practical Approach aims to aid scientists in their pursuit of forwarding this vital field of study.
Inhaltsverzeichnis zu „Genotoxicity and DNA Repair “
Part I: Genotoxicity Assays1. Ames Test (Bacterial Reverse Mutation Test): Why, When, and How to Use
Araceli Pillco and Eduardo de la Peña
2. The Ames II and Ames MPF Penta I Assay: A Liquid Microplate Format Modification of the Classic Ames Test
Sini Flückiger-Isler and Markus Kamber
3. Revised Procedure of the Bacterial Reverse Mutation Test for Genotoxic Evaluation of Nanoparticles
Christophe Pagnout, Stéphane Jomini, and Pascale Bauda
4. The Control of Hydrophobic Compound Exposure in In Vitro Tests for Genotoxicity
Kilian E.C. Smith
5. The In Vitro Micronucleus Assay and FISH Analysis
Lucia Migliore, Sebastiano Di Bucchianico, and Chiara Uboldi
6. The In Vivo Rodent Micronucleus Test
Edson Luis Maistro
7. Chromosomal Aberration Test Utilities In Vitro and In Vivo
Ana Paula A. Guimarães, Adriana C. Guimarães, Diego Á. Alcântara, Luiz Raimundo Cunha, Patrícia L. Lima, Marne C. Vasconcellos, Raquel C. Montenegro, Bruno M. Soares, Marucia M. Amorim, and Rommel R. Burbano
8. Chromosome Painting of Mouse Peripheral Blood and Spleen Tissues
Dayton M. Petibone, James D. Tucker, and Suzanne M. Morris
9. T-Cell Receptor Mutation Assay for Monitoring Human Genotoxic Exposure
Seishi Kyoizumi
10. The Human RBC PIG-A Gene Mutation Assay
Vasily N. Dobrovolsky and Robert H. Heflich
11. The Applicable Use of the HPRT Gene Mutation Assay as a Practical Tool in Mutagenesis and DNA Repair Studies
Zoulikha M. Zaïr and George E. Johnson
12. The Comet Assay: High Throughput Use of FPG
Amaya Azqueta and Andrew R. Collins
13. The Comet Assay In Vivo in Humans
Carla Costa and João Paulo Teixeira
14. Analysis of Nanoparticle-Induced DNA Damage by the Comet Assay
Julia Catalán, Satu Suhonen, Anna Huk, and Maria Dusinska
15. The Comet Assay in Drosophila: Neuroblast and Haemocyte Cells
L. María Sierra, Erico R. Carmona, Leticia Aguado, and Ricard Marcos
16. The SMART Assays of Drosophila: Wings
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and Eyes as Target Tissues
Ricard Marcos, L. María Sierra, and Isabel Gaivão
17. Testing the Genotoxic Potential of Nanomaterials Using Drosophila
Mohamed A. Abdalaziz, Balasubramanyam Annangi, and Ricard Marcos
18. Transgenic Rodent Gene Mutation Assay in Somatic Tissues
John D. Gingerich, Lynda Soper, Christine L. Lemieux, Francesco Marchetti, and George R. Douglas
19. The Mouse Lymphoma Assay
Tao Chen, Xiaoqing Guo, and Martha M. Moore
20. Bhas 42 Cell Transformation Assay for Genotoxic and Non-Genotoxic Carcinogens
Kiyoshi Sasaki, Anna Huk, Naouale El Yamani, Noriho Tanaka, and Maria Dusinska
Part II: DNA Repair Assays
21. Methods for Measuring DNA Repair: Introduction and Cellular Repair
Andrew R. Collins and Amaya Azqueta
22. A Standardized Protocol for the In Vitro Comet-Based DNA Repair Assay
Jana Slyskova, Sabine A.S. Langie, Isabel Gaivão, Andrew R. Collins, and Amaya Azqueta
23. Use of the Comet Assay to Study DNA Repair in Drosophila melanogaster
Isabel Gaivão, Rubén Rodríguez, and L. María Sierra
24. Use of RNA Interference to Study DNA Repair
Elise Fouquerel, Jianfeng Li, Andrea Braganza, Zhongxun Yu, Ashley R. Brown, Xiao-hong Wang, Sandy Schamus, David Svilar, Qingming Fang, and Robert W. Sobol
Part III: Others
25. The LacZ Plasmid-Based Transgenic Mouse Model: An Integrative Approach to Study the Genotoxicity of Nanomaterials
Henriqueta Louro, Miguel Pinto, Nádia Vital, Ana M. Tavares, Pedro M. Costa, and Maria João Silva
Ricard Marcos, L. María Sierra, and Isabel Gaivão
17. Testing the Genotoxic Potential of Nanomaterials Using Drosophila
Mohamed A. Abdalaziz, Balasubramanyam Annangi, and Ricard Marcos
18. Transgenic Rodent Gene Mutation Assay in Somatic Tissues
John D. Gingerich, Lynda Soper, Christine L. Lemieux, Francesco Marchetti, and George R. Douglas
19. The Mouse Lymphoma Assay
Tao Chen, Xiaoqing Guo, and Martha M. Moore
20. Bhas 42 Cell Transformation Assay for Genotoxic and Non-Genotoxic Carcinogens
Kiyoshi Sasaki, Anna Huk, Naouale El Yamani, Noriho Tanaka, and Maria Dusinska
Part II: DNA Repair Assays
21. Methods for Measuring DNA Repair: Introduction and Cellular Repair
Andrew R. Collins and Amaya Azqueta
22. A Standardized Protocol for the In Vitro Comet-Based DNA Repair Assay
Jana Slyskova, Sabine A.S. Langie, Isabel Gaivão, Andrew R. Collins, and Amaya Azqueta
23. Use of the Comet Assay to Study DNA Repair in Drosophila melanogaster
Isabel Gaivão, Rubén Rodríguez, and L. María Sierra
24. Use of RNA Interference to Study DNA Repair
Elise Fouquerel, Jianfeng Li, Andrea Braganza, Zhongxun Yu, Ashley R. Brown, Xiao-hong Wang, Sandy Schamus, David Svilar, Qingming Fang, and Robert W. Sobol
Part III: Others
25. The LacZ Plasmid-Based Transgenic Mouse Model: An Integrative Approach to Study the Genotoxicity of Nanomaterials
Henriqueta Louro, Miguel Pinto, Nádia Vital, Ana M. Tavares, Pedro M. Costa, and Maria João Silva
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Bibliographische Angaben
- 2014, 2014, XII, 483 Seiten, 42 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: L. María Sierra, Isabel Gaivão
- Verlag: Springer, Berlin
- ISBN-10: 1493910671
- ISBN-13: 9781493910670
Sprache:
Englisch
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