Germ Cell Protocols

Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos.- Analysis of Germline Chromatin Silencing by Double-Stranded RNA-Mediated Interference (RNAi) in Caenorhabditis elegans.- cDNA Subtraction and Cloning in the Field of Trophoblast/Placental Development.- PCR-Based Cloning and Differential Screening of RNAs from Xenopus Primordial Germ Cells.- Differentiating Embryonic Stem Cells into Embryoid Bodies.- The Derivation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation.- Using Multiphoton Excitation to Explore the Murky Depths of Developing Embryos.- Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes.- A Method for Producing Cloned Pigs by Using Somatic Cells as Donors.- Pronuclear Microinjection of Mouse Zygotes.- Parthenogenesis and Nuclear Transfer in Rabbit Oocytes.- Nuclear Transfer in Cattle.- In Vitro Fertilization and Embryo Transfer in Felids.- Production of Chimeric Chickens.- Production of Haploid and Diploid Androgenetic Zebrafish.- Construction and Detection of Fluorescent, Germline Transgenic Zebrafish.- Production of Zebrafish Germline Chimeras from Cultured Cells.- Studying Nuclear Reprogramming with Cell Hybrids.- Cytoplasmic Transfer in Mammalian Eggs and Embryos.- Conventional Technologies for Cryopreservation of Human Oocytes and Embryos.- Vitrifying and Warming of Human Oocytes, Embryos, and Blastocysts.- Microfluidic Removal of Cumulus Cells from Mammalian Zygotes.- Mammalian Embryo Culture in a Microfluidic Device.- Application of Alginate Gels to the Study of Mammalian Development.- Using TUNEL in Combination with an Active Caspase-3 Immunoassay to Identify Cells Undergoing Apoptosis in Preimplantation Mammalian Embryos.