Handbook for Rhizobia

IV. Inoculant Technology.- 25. Producing Broth Cultures in Simple Glass Fermentors.- 26. Producing Inoculum in a Steel Fermentor.- 27. Preparing a Range of Carrier Materials and Producing Inoculants.- 28. Preparing Inoculants Using Diluted Cultures of Rhizobia and Presterilized Peat.- 29. Testing the Survival of Rhizobia on Inoculated Seeds.- Additional References and Recommended Reading.- V. Genetic Techniques for Rhizobia.- 30. Analyzing Plasmid Profiles of Rhizobium spp. by a Modified Eckhardt Vertical Gel Electrophoresis Procedure.- 31. Isolating and Purifying Genomic DNA of Rhizobia Using a Large-Scale Method.- 32. Isolating and Purifying Genomic DNA of Rhizobia Using a Rapid Small-Scale Method.- 33. Digesting Genomic DNA of Rhizobia with Restriction Endonucleases.- 34. Separating Restriction Fragments of Genomic DNA by Horizontal Agarose Gel Electrophoresis.- 35. Transferring Electrophoretically Separated DNA from Agarose Gels to a Membrane by Southern Blotting.- 36. Preparing a DNA Probe for Detecting the nif Genes on Symbiotic Plasmids of Rhizobium spp.- 37. Incorporating a Nonradioactive Label into a DNA Probe by Nick Translation.- 38. Using a Nonradioactively Labeled nifKDH Gene Probe to Locate Complementary Sequences of Rhizobial DNA Immobilized on Membranes.- Additional References and Recommended Reading.- VI. Appendices.- 1. Characteristics of the Subfamilies of Legumes.- 2. The Nodule Preservation Vial.- 3. Bacterial Growth Media and Plant Nutrient Solutions.- 4. Reagents and Buffers.- 5. Molecular Biology Reagents and Buffers.- 6. McFarland Nephelometer Barium-Sulfate Standards.- 7. Preparing Seedling-Agar Slants and NifTAL-Tubes for Culturing Small-Seeded Legumes.- 8. Building a Rack for Growth Pouches.- 9. Recommendations of Legumes and Growth Systems for Authentication.- 10. Seed Surface Sterilization and Germination.- 11. Preparing Leonard Jars.- 12. Injecting and Bleeding Rabbits.- 13. The Indirect Fluorescent Antibody Technique.- 14. Additional Information on the Plant Infection Count.- 15. The Acetylene Reduction Assay for Measuring Nitrogenase Activity.- 16. Methods for Determining Lime Requirements of Acid Soils.- 17. Analysis of Variance for a Rhizobial Strain Selection Experiment.- 19. Replicators and Microtiter Plates.- 20. Seed Inoculation Procedures.- 21. Determining Field Capacity of Field Soil.- 22. The Simple Transfer Chamber.- 23. Freeze Drying Cultures of Rhizobia.- 24. Source of Rhizobia.- 25. Absorption of Antisera.- Supplemental Reading List.