Handbook of ELISPOT

Inhaltsverzeichnis zu „Handbook of ELISPOT “

Preface... Table of Contents... Contributing Authors...
1. Challenges in Developing Protein Secretion Assays at a Single-Cell Level Yoshitaka Shirasaki and Osamu Ohara
2. To Count of Not to Count: ELISPOT Evaluation Revisited Sylvia Janetzki
3. Essential Controls for ELISpot Assay Jodi Hagen and Alex Kalyuzhny
4. Automatic Search of Spots and Color Classification in ELISPOT Assay Sergey S. Zadorozhny and Nikolai N. Martynov
5. Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages Jodi Hanson, Diana R. Roen, and Paul V. Lehmann
6. Detection of Cross-Reactive B Cells Using the FluoroSpot Assay Peter Jahnmatz and Niklas Ahlborg
7. Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations Diana R. Roen, Jodi Hanson, and Paul V. Lehmann
8. Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays Richard Caspell and Paul V. Lehmann
9. Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm Alexey Y. Karulin, Zoltán Megyesi, Richard Caspell, Jodi Hanson, and Paul V. Lehmann
10. Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X(TM) Suite Zoltán Megyesi, Paul V. Lehmann, and Alexey Y. Karulin
11. B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells Haw Hwai, Yi-Ying Chen, and Siang-Jong Tzeng
12. Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOT Alison J. Johnson, Steven C. Kennedy, Tony W. Ng, and Steven A. Porcelli
13. ELISPOT-Based "Multi-Color FluoroSpot" to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections Paulina Andrade, Josefina Coloma, and Eva Harris
14. Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses Chandima Jeewandara, Graham S. Ogg, and Gathsaurie Neelika