Hematopoietic Stem Cell Protocols
(Sprache: Englisch)
This revised edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. The book...
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This revised edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. The book provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations, and offers a variety of transplantation approaches to measure HSC function in vivo. This is a much needed technical resource in the critically important field of stem cell investigation.
Klappentext zu „Hematopoietic Stem Cell Protocols “
The hematopoietic stem cell (HSC) field has rapidly grown in the past several years as new technologies have been developed and the older tried and true methods have been used in new ways. Major advances in the isolation of HSC and progenitor subsets have been sparked by the gene expression array technologies, leading to the identification of new markers for prospective isolation. The first edition of Hematopoietic Stem Cell Protocols is a very thorough resource covering historically the basic techniques that have been published extensively over many years. To put together, a follow-up to this excellent book has been a hard act to follow. The intent of this book is to be distinct and not duplicate but to build on the strong basic assays and include updated approaches for the combination of phenotypic and functional analyses. This second edition of Hematopoietic Stem Cell Protocols thus aims to provide timely protocols developed in the HSC field since the last comprehensive review in this series. The approaches described in this book should be readily useful for the basic science researcher, especially those familiar with the use of mouse genetic models. The chapters in this book are geared toward the laboratory-based researcher and the development of pre-clinical studies to apply HSCs toward treating blood diseases more effectively.
Inhaltsverzeichnis zu „Hematopoietic Stem Cell Protocols “
Overview.- The Expanding Tool Kit for Hematopoietic Stem Cell Research.- Stem Cell Enrichment and Analysis.- Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties.- Mobilization of Hematopoietic Stem and Progenitor Cells in Mice.- Transmigration of Human CD34+ Cells.- Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells.- Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry.- In Vitro Assays and Differentiation.- In Vitro Hematopoietic Differentiation of Murine Embryonic Stem Cells.- Hematopoietic Development of Human Embryonic Stem Cells in Culture.- In Vitro Human T Cell Development Directed by Notch-Ligand Interactions.- In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C.- Transplantation Assays for Mouse and Human HSC.- Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection.- Hematopoietic Stem Cell Transplant into Non-Myeloablated W/W v Mice to Detect Steady-State Engraftment Defects.- Isolation and Functional Characterization of Side Population Stem Cells.- Transplantation of Chimeric Fetal Liver to Study Hematopoiesis.- Immunodeficient Mouse Models to Study Human Stem Cell-Mediated Tissue Repair.- Genetic Modification of HSCs and Imaging Engraftment.- Retroviral Transduction of Murine Hematopoietic Stem Cells.- Lentiviral Gene Transduction of Mouse and Human Stem Cells.- Retroviral Integration Site Analysis in Hematopoietic Stem Cells.- A Detailed Protocol for Bacterial Artificial Chromosome Recombineering to Study Essential Genes in Stem Cells.- Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models.
Autoren-Porträt
The field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. Updating and building upon the thorough first edition's foundation, this volume provides up-to-date protocols developed in the HSC field. A team of leading researchers supply the volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. An introductory chapter also hints at potential techniques that may develop in the future. Following the format of the successful Methods in Molecular Biology series, each protocol offers step-by-step laboratory instructions, lists of the necessary equipment, and tips on troubleshooting and avoiding known pitfalls. Essential for the laboratory-based researcher, this book is a much needed technical resource in the critically important field of stem cell investigation.
Bibliographische Angaben
- 2010, 2nd Edition., XIV, 312 Seiten, Maße: 15,5 x 23,5 cm, Kartoniert (TB), Englisch
- Herausgegeben: Kevin D. Bunting
- Verlag: Springer, Berlin
- ISBN-10: 161737816X
- ISBN-13: 9781617378164
Sprache:
Englisch
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