Hematopoietic Stem Cell Protocols

The Hematopoietic Stem Cell Landscape.- Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow.- Single-Cell PCR Profiling of Gene Expression in Hematopoiesis.- Hematopoietic Stem and Progenitor Cell Mobilization in Mice.- Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells.- Flow Cytometry Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells.- Gene Expression Profiling of Hematopoietic Stem Cells (HSC).- Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells.- DNA Methylation Profiling of Hematopoietic Stem Cells.- Metabolic Characterization of Hematopoietic Stem Cells.- Nano Proteomic Assays on Hematopoietic Stem Cells.- Hematopoietic Differentiation of Pluripotent Stem Cells in Culture.- Ex vivo Assays to Study Self-Renewal, Long-Term Expansion and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells.- Ex vivo Expansion of Murine and Human Hematopoietic Stem Cells.- Using Microfluidics to Investigate Hematopoietic Stem Cell and Micro niche Interactions at the Single Cell Level.- Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells.- Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull.- Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development.- Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells.- Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells.- Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells.- High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies.- Barcoded Vector Libraries and Retro- or Lentiviral Barcoding of Hematopoietic Stem Cells.- Barcoded Vector Libraries and Retro- or Lentiviiral Barcoding of Hematopoietic StemCells.- Using Microfluidics