Herpes Simplex Virus

Tour de Herpes: Cycling through the Life and Biology of HSV-1.- Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology.- Herpes Simplex Virus Growth, Preparation, and Assay.- Engineering HSV-1 Vectors for Gene Therapy.- Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors.- HSV-1 Amplicon Vectors as Genetic Vaccines.- oHSV Genome Editing by Means of galK Recombineering.- Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein.- CRISPR/Cas9-Based Genome Editing of HSV.- Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescent In Situ Hybridization (FISH).- Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for use in High-Throughput Sequencing.- HSV Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo.- Phenotypic and Genotypic Testing of HSV-1 and HSV-2Resistance to Antivirals.- Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation.- Characterization of Extracellular HSV-1 Virions by Proteomics.- Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry.- Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells.- Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot.- BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks.- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy.- Transmission Immuno-Electron Microscopy of Herpes Simplex Virus-1 Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane.- Multi-Fluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication.- Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination.- Expression,