High-Resolution Imaging of Cellular Proteins

Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture.- Antibody Production with Synthetic Peptides.- Production and Purification of Polyclonal Antibodies.- Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2 and Streptavidin.- Helper Dependent Adenoviral Vectors and their use for Neuroscience Applications.- Localizing Proteins in Fixed Giardia Lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy.- Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells.- Using FRAP Or FRAPA to Visualize the Movement of Fluorescently-Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses.- Bimolecular Fluorescence Complementation (BiFc) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells.- Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging.- Imaging SynapticVesicle Exocytosis-Endocytosis with pH Sensitive Fluorescent Proteins.- Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas.- Pre-Embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells.- Immunoelectron Microscopy of Cryofixed and Freeze- Substituted Plant Tissues.- Immunoelectron Microscopy of Cryofixed Freeze Substituted Yeast Saccharomyces cerevisiae.- Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes.- Pre-Embedding Nanogold Silver and Gold Intensification.- Post-Embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based On Heat-Induced Antigen Retrieval.- Pre- and Post-Embedding Immunogold Labeling of Tissue Sections.- Immuno-Gold Labelling for Scanning Electron Microscopy.- Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope.- Functional Nanoscale Imaging Of Synaptic

"This is a collection of timely techniques and methods that have been key for the development of imaging at the structural and ultrastructural level. ... The intended audience is cell biologists and all scientists working with imaging techniques to localize protein expression at a cellular and subcellular level. ... Each chapter ends with a list of references. Graphics and high-quality figures are displayed where needed." (Michele Fornaro, Doody's Book Reviews, July, 2017)