Immune Receptors

Screening for Ligands of C-Type Lectin-Like Receptors.- Yeast Surface Display of Lamprey Variable Lymphocyte Receptors (VLR).- Identification of Scavenger Receptor Ligands.- Construction, Expression, and Purification of Chimeric Protein Reagents Based on Immunoglobulin Fc Regions.- Innate Immune Receptors for Nucleic Acids.- Analysis of Receptor-Ligand Interactions by Surface Plasmon Resonance.- Cell-Based Reporter Assay to Analyze Activation of Nod1 and Nod2.- Determining Fc RI Diffusional Dynamics Via Single Quantum Dot Tracking.- Ratiometric Analysis of Subcellular Recruitment of Fc Receptors During Phagocytosis.- Assessment of the Recycling of the Membrane-Bound Chemokine, CX3CL1.- Measuring Immune Receptor Mobility by Fluorescence Recovery after Photobleaching.- Probing the Plasma Membrane Structure of Immune Cells through the Analysis of Membrane Sheets by Electron Microscopy.- Rapamycin-Based Inducible Translocation Systems for Studying Phagocytosis.- Micropatterned Ligand Arrays to Study Spatial Regulation in Fc Receptor Signaling.- CELLISA: Reporter Cell-Based Immunization and Screening of Hybridomas Specific for Cell Surface Antigens.- Use of Colloidal Silica-Beads for the Isolation of Cell-Surface Proteins for Mass Spectrometry-Based Proteomics.- Transfection-Based Genomic Readout for Identifying Rare Transcriptional Splice Variants.- Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System.- Characterizing Immune Receptors from New Genome Sequences.