Influenza Virus

Understanding Influenza.- Clinical Diagnosis of Influenza.- Influenza A Virus Genetic Tools - From Clinical Sample to Molecular Clone.- Propagation and Titration of Influenza Viruses.- Purification and Proteomics of Influenza Virions.- Haploid Screening for the Identification of Host Factors in Virus Infection.- Phenotypic Lentivirus Screens to Identify Antiviral Single Domain Antibodies.- Deciphering Virus Entry with Fluorescently-Labeled Viral Particles.- Quantitative RT-PCR Analysis of Influenza Virus Endocytic Escape.- Single Molecule Sensitivity RNA FISH Analysis of Influenza Virus Genome Trafficking.- Correlative Light and Electron Microscopy (CLEM) and 3D Methods to Study Architecture of Virus-Infected Cells.- Correlative Light and Electron Microscopy (CLEM) of Influenza Virus Entry and Budding.- Influenza Virus-Liposome Fusion Studies using Fluorescence Dequenching and Cryo-Electron Tomography.- Metal-Tagging Transmission Electron Microscopy and Immunogold Labelling on Tokuyasu Cryosections to Image Influenza A Virus Ribonucleoprotein Transport and Packaging.- Live Imaging of Influenza Viral Ribonucleoproteins using Light-Sheet Microscopy.- Purification of Unanchored Polyubiquitin Chains from Influenza Virions.- Measurement of Influenza Virus Polymerase Activity.- In vitro models to study influenza virus and Staphylococcus aureus super-infection on a molecular level.- Infection of Cultured Mammalian Cells with Aerosolized Influenza Virus.- Animal Models in Influenza Research.- Measuring Influenza Virus Infection using Bioluminescent Reporter Viruses for in vivo Imaging and in vitro Replication Assays.- Selection of Antigenically Advanced Variants of Influenza Viruses.- Assessment of Influenza Virus Hemagglutinin Stalk-specific Antibody Responses.- Analyses of Cellular Immune Responses from Ferrets Following Influenza Virus