Innate DNA and RNA Recognition
Methods and Protocols
(Sprache: Englisch)
Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include...
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Klappentext zu „Innate DNA and RNA Recognition “
Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources.
Inhaltsverzeichnis zu „Innate DNA and RNA Recognition “
Detection of RNA Modifications by HPLC Analysis and Competitive ELISA.- Enzymatic Synthesis and Purification of a Defined RIG-I Ligand.- Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis.- Isolation of RIG-I-associated RNAs from Virus Infected Cells.- Structure Modeling of Toll-like Receptors.- Nucleic Acid Recognition in Dendritic Cell.- Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems.- Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro.- In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes.- Mapping of Optimal CD8 T Cell Epitopes.- A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium and Viral Infection.- MicroRNA Methodology: Advances in miRNA-technologies.- Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR).- Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus.- Induction and Analysis of Nephrotoxic Serum Nephritis in Mice.- Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice.- Bifunctional siRNAs for Tumor Therapy.
Bibliographische Angaben
- 2016, Softcover reprint of the original 1st ed. 2014, 195 Seiten, 16 farbige Abbildungen, Maße: 17,8 x 25,4 cm, Kartoniert (TB), Englisch
- Herausgegeben: Hans-Joachim Anders, Adriana Migliorini
- Verlag: Springer, Berlin
- ISBN-10: 1493945831
- ISBN-13: 9781493945832
Sprache:
Englisch
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