Isolation of Plant Organelles and Structures

The Isolation of Plant Organelles and Structures in the Post-Genomics Era.- Approaches to Characterize Organelle, Compartment, or Structure Purity.- Isolation of Nuclei and Nucleoli.- Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts.- Isolation of Chromoplasts and Sub-Organellar Compartments from Tomato and Bell Pepper Fruit.- Leucoplast Isolation and Subfractionation.- Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes.- Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane.- Isolation of Vacuoles and the Tonoplast.- Isolation of Endoplasmic Reticulum and Its Membrane.- Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings.- Isolation of Autolysosomes from Tobacco BY-2 Cells.- Isolation of Protein Storage Vacuoles and Their Membranes.- Isolation of the Cell Wall.- Isolation of Plasmodesmata.- Isolation of Plasma Membrane and Plasma Membrane Microdomains.- Enrichment of the Plant Cytosolic Fraction.- Isolation of Apoplast.- Isolation of Cytosolic Ribosomes.- Isolation of Plastid Ribosomes.- Isolation of Mitochondrial Ribosomes.- Isolation of Microtubules and Microtubule-Associated Proteins.- Isolation of Actin and Actin Binding Proteins.- Purification of 26S Proteasomes and Their Sub-Complexes from Plants.