Kidney Development

Dissecting and Culturing and Imaging the Mouse Urogenital System.-In Vitro Culture of Embryonic Kidney Rudiments and Isolated Ureteric Buds.-In vitro Induction of Nephrogenesis in Mouse Metanephric Mesenchyme with Lithium Introduction and Ureteric Bud Recombination.-Live Imaging of the Developing Mouse Mesonephros.-Organotypic Culture of the Urogenital Tract.-Live Imaging Kidney Development in Zebrafish.-Analysis of 3D Branching Pattern: Hematoxylin and Eosin Method.-Three-Dimensional Imaging of Fetal Mouse Kidneys.-Analysis of Native Kidney Structures in Three Dimensions.-Estimating Nephron Number in the Developing Kidney using the Physical Disector/Fractionator Combination.-An Immunofluorescence Method to Analyze the Proliferation Status of Individual Nephron Segments in the Xenopus Pronephric Kidney.-Dissociation of Embryonic Kidney Followed by Re-aggregation as a Method for Chimeric Analysis.-Analysis of Migration in Primary Ureteric Bud Epithelial Cells.-Investigating Primary Cilia in Cultured Metanephric Mesenchymal Cells.-Making Immortalized Cell Lines from Embryonic Mouse Kidney.-Engineered Tissues to Quantify Collective Cell Migration During Morphogenesis.-Access and use of the GUDMAP Database of Genitourinary Development.- Isolation of High Quality RNA from Embryonic Kidney and Cells.-Laser Capture.-Use of in Situ Hybridization to Examine Gene Expression in the Embryonic, Neonatal and Adult Urogenital System.-Detection of beta-galactosidase Activity: X-gal Staining.-Fluorescent Immunolabeling of Embryonic Kidney Samples.-Immunohistochemical Staining of dpERK Staining During Early Kidney Development.-SensingBMP Pathway Activity by Immune Detection of Phosphorylated R-Smad Proteins in Mouse Embryonic Kidney.-Analysis of in vivo Transcription Factor Recruitment by Chromatin Immunoprecipitation (ChIP) of Mouse Embryonic Kidney.-siRNA-mediated RNA Interference in Embryonic Kidney Organ Culture.-Morpholino-mediated Gene Knockdown in Mammalian Organ