Kinase Screening and Profiling

HTRF Kinase Assay Development and Methods in Inhibitor Characterization.- Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening.- Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy.- Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase and PI5P4K Lipid Kinase.- Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity.- Measuring Activity of Phosphoinositide Lipid Kinases Using a Bioluminescent ADP-Detecting Assay.- A High-Throughput Radiometric Kinase Assay.- A High Content Assay to Screen for Modulators of EGFR Function.- Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody Microarrays.- From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors.- Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells.- Characterization of an Engineered Src Kinase to Study Src Signaling and Biology.- Screening One-Bead-One-Compound Peptide Libraries for Optimal Kinase Substrates.- Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and Macroarrays.- Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries.