Melatonin

Two-Dimensional Thin Layer Chromatography of Melatonin and Related Compounds.- Detection of Melatonin in Tissue Samples.- Measuring Dim Light Melatonin Onset in Humans.- Measuring Urinary 6-sulphatoxymelatonin in Humans.- MEL-Index: Estimation of Tissue Melatonin Levels using Gene Expression Data.- Melatonin Synthesis Enzymes Activity: Radiometric Assays for AANAT, ASMT, and TPH.- Pinealectomy in Rats.- Surgical Techniques and Nuances for Superior Cervical Ganglionectomy and Decentralization in Rats.- Pineal Microdialysis.- Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland.- Pineal Cells Dissociation and Culture: Isolated Pinealocytes, Isolated Astrocytes, and Co-Culture.- Pineal Gland Culture.- Preparation of Single Pineal Cells.- RNA Sequencing of Single Pineal Cells.- siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells.- Analysis of the Human Pineal Proteome by Mass Spectrometry.- The Pineal Transcriptome Webpage.- 2-[125I]iodomelatonin and [3H]melatonin Binding Assays for Melatonin Receptors.- Alternative Ligands at Melatonin Receptors.- GTP S Binding Assay for Melatonin Receptors in Mouse Brain Tissue.- MT1 Melatonin Receptor Reconstitution in Nanodiscs.- Functional Investigation of Melatonin Receptors Activation by Homogenous cAMP Assay.- Functionality of Melatonin Receptors: Internalization.- Functionality of Melatonin Receptors: Recruitment of beta-arrestin at MT1.- MT1 Receptor Signaling Pathways by Impedance Measurement.- Measuring Protein-Protein Interactions of Melatonin Receptors by Bioluminescence Resonance Energy Transfer (BRET).- Non-Radioactive In Situ Hybridization of MT1 Melatonin Receptor for the Identification of Melatonin Target Cells.- Beta-Galactosidase as a Transgenic Reporter for the Mapping and Phenotyping of MT1 and MT2 Melatonin Receptor Expressing