Metalloproteins
Methods and Protocols
(Sprache: Englisch)
Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of...
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Klappentext zu „Metalloproteins “
Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.
Inhaltsverzeichnis zu „Metalloproteins “
Advances in Research on Metalloproteins.- Purification of O2-Sensitive Metalloproteins.- Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources.- Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins.- Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers.- Electrochemistry of Metalloproteins - Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1.- FTIR Spectroscopy of Metalloproteins.- Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy.- A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds.- Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR).- Mössbauer Spectroscopy.- X-Ray Absorption Spectroscopy of Metalloproteins.- X-Ray Crystallographic Studies of Metalloproteins.- Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds.- Density Functional Theory - Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups.
Bibliographische Angaben
- 2014, 2014, X, 299 Seiten, 57 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Juan C. Fontecilla-Camps, Yvain Nicolet
- Verlag: Springer, Berlin
- ISBN-10: 1627037934
- ISBN-13: 9781627037938
- Erscheinungsdatum: 18.03.2014
Sprache:
Englisch
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