Methods in Actinobacteriology

Antismash: Secondary Metabolite Genome Mining Pipeline.- Biosynthetic Gene Cluster Analysis in Actinobacterial Genera Streptomyces spp..- PCR based Determination of Secondary Metabolite Genes in Actinobacterial Cultures. Molecular Mass Determination of Bacteriocin by SDS-PAGE analysis.- MALDI-TOF Analysis of Actinobacterial Peptides with Respect to MASCOT Database.- Protocols for Preclinical Evaluation and Molecular Docking of Antimicrobial Compounds from Streptomyces sp.- Drug Likeliness Evaluation, ADME-Toxicity Investigation, Docking modes between the Ligand and the target Enzyme and Active Site Prediction.- Energy-based Pharmacophore Hypothesis Combined with Molecular Simulation Protocol for The Screening Of Bioactive Compounds From the Class of Actinobacteria.- Receptor cavity-based approach combined with Autodock protocol for the screening of antiviral compounds from Streptomyces sp..- Pharmacophore Based Hypothesis Combined with Molecular Docking Protocol for the Screening Of Anti-Cancer Compounds from Streptomyces sp..- Methods of Identification and Validation of Drug Target.- Transcriptome profiles of Streptomyces sp..- Culture of Actinobacteria, Isolation, and Characterization of their Bioactive Compounds.- Miniaturized Production of Bioactive Extracts from Actinobacteria.- Screening, Characterization, and Identification of Antibacterial Compounds From Actinobacteria.- Isolation, Identification, and Screening of Polyene Antifungal Compound Producing Streptomyces sampsonii MDCE7 from agroforestry soil.- Screening of Actinobacterial Cultures for Antimycobacterial Activity using Mycobacterium smegmatis.- Screening of Actinobacterial Extracts/Compounds for Antimycobacterial Activity by Luciferase Reporter Phage (LRP) Assay.- Screening of Actinobacteria for Anti-TB Activity by MicroplateAlamarBlue Assay (MABA).- Screening of Actinobacteria for anti-TB Activity by Agar Dilution Assay.- Antiplasmodial activity of Halophilic Actinobacteria.- An invitroAntiamoebic Activity of Actinobacteria.- Screening for Antiviral Activity: MTT Assay.- Screening for Anticancer Activity: 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium (MTT) Assay.- Screening for Anticancer Activity: Dual Staining Method.- Screening for Anticancer Activity: Neutral Red Uptake Assay.- Screening for Anticancer Activity: Lactic Acid Dehydrogenase Assay.- Screening for Anticancer Activity: DNA Fragmentation Assay.- Screening for Anticancer Activity: Trypan Blue Exclusion Assay.- In vitro Evaluation of Antimitotic Properties Of Actinobacterial Extracts Using Onion Root Tip Assay.- Screening for Antioxidant Activity: Diphenylpicrylhydrazine (DPPH) Assay.- Screening for Antioxidant Activity: Nitric Oxide Scavenging Assay.- Screening for Antioxidant Activity: Metal Chelating Assay.- Screening for Antioxidant Activity: Total Antioxidant Assay.- Screening for Antioxidant Activity: Hydrogen Peroxide Scavenging Assay.- In vitro Evaluation Of Actinobacterial Extracts For Anti Inflammatory Properties.- Screening and production of anti freeze Proteins from Actinobacteria.- In vitro Evaluation of Actinobacterial Extracts for Immunomodulatory Properties.- Screening of Actinobacteria for Enzyme Inhibitor Activity.- Screening of Actinobacteria for Quorum Sensing Inhibition.- Screening of Actinobacterial Extracts for Anti-biofilm Activity.- Screening of Actinobacteria for Microbial Induced Calcium Precipitation (MICP).- Immobilization of Actinobacterial Cells - Sodium Alginate and Calcium Chloride Method.- Production of Actinobacteria Amylase by Fermentation in Solid State Using Residues Of Licuri Palm (Syagruscoronata).- Production Of Lipase By Actinobacteria.- Isolation And Cultivation Of Actinobacteria By Submerged Fermentation For The Production Of Keratinase.- Screening of Cellulase from Actinobacteria.- Screening of Pectinase from Actinobacteria.- Screening of Protease from Actinobacteria.- Screening and analysis of Actinobacterial Bioherbicides For Weed

Management.- In vitro Assessment of Actinobacteria for Survivability Under Simulated Gastro-Intestinal Transit.- In vitro Assessment of Actinobacteria for Survivability Under Simulated Gastro-Intestinal Transit.- Screening of Actinobacterial Probiotics by Anti-Pathogenic Activity Test.- Screening and Analysis of Probiotic Actinobacteria in Poultry Farming.- Vitamin B12 Producing Actinobacteria as Probiotics for Poultry Production.- Qualitative and Quantitative Estimation of Phosphate Solubilizing Actinobacteria.- Estimation of Nitrogen Production by Actinobacteria.- Screening of Actinobacteria for Siderophore Production.- Isolation and Screening of Symbiotic Actinobacteria From Root Nodules of Actinorhizal plant Casuarina sp.- Biocontrol Activity of Actinobacteria Against Plant Pathogens.- An In vitro nematicidalactivity ofactinobacteria- Juvenile mortality and Egg hatching inhibition.- Estimation of Auxin Production by Actinobacteria.- Screening of Actinobacteria for Mosquitocidal Activity.- Extraction, Characterization, and Identification of Odorous Metabolites from Streptomyces.- Screening of Actinobacteria for Biosurfactant Production.- Optimization and Characterization of Biosurfactant from Streptomyces.- Production of Vitamin B12from Streptomyces species.- Screening of Microbes for the Production of Pigment (Melanin).- Exploration and Characterization ofMelanin Pigment Produced by Actinomycetes.- Isolation and Production of Prodigiosin Pigments from Streptomyces spp..- Aerobic and Anaerobic Decolourization of Textile Dyes Using Actinobacteria.- Bioleaching of heavy metals from e-waste using actinobacteria.- Biosynthesis and Characterization of Silver Nanoparticles From Actinobacteria.- Biosynthesis and Characterization of Gold Nanoparticles from Actinobacteria.- Antimicrobial Activity of Extracellular Green-Synthesized Nanoparticles By Actinobacteria.- Antibiofilm Activity of Extracellular Green-Synthesized Nanoparticles By Actinobacteria.- Cytotoxic Activity of Extracellular Green Synthesized Nanoparticles by Actinobacteria.- Sporicidal Activity of Extracellular Green-Synthesized Nanoparticles by Actinobacteria.