Microbial Metabolic Engineering

Screening for Cellulases with Industrial Value and their use in Biomass Conversion.- Reversal of NAD(P)H Cofactor Dependence by Protein Engineering.- Quantifying Plasmid Copy Number to Investigate Plasmid Dosage Effects Associated with Directed Protein Evolution.- High Isoprenoid Flux Escherichia coli as a Host for Carotenoids Production.- Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: Deletion of gal7.-Creation of New Metabolic Pathways or Improvement of Existing Metabolic Enzymes by in vivo Evolution in Escherichia coli.- Bioluminescent Reporter Genes for Promoter Discovery.-Recombination-based DNA Assembly and Mutagenesis Methods for Metabolic Engineering.- Ethanol-tolerant Gene Identification in Clostridium thermocellum using Pyro-resequencing for Metabolic Engineering.-Use of Proteomic Tools in Microbial Engineering for Biofuel Production.-Metabolic Engineering of Antibiotic-producing Actinomycetes using in vitro Transposon Mutagenesis.-Use FACS Sorting in Metabolic Engineering of Escherichia coli for Increased Peptide Production.-Using Flux Balance Analysis to Guide Microbial Metabolic Engineering.-Using an Advanced Microfermentor System for Strain Screening and Fermentation Optimization.-Rapid Strain Evaluation using Dynamic DO-stat fed-batch Fermentation Under Scale-down Conditions.- Preparation and Evaluation of Lignocellulosic Biomass Hydrolysates for Growth by Ethanolgenic Yeasts.-Engineering Whole-cell Biosensors with No Antibiotic Markers for Monitoring Aromatic Compounds in theEnvironment.-Metabolic Engineering for Acetate Control in Large Scale Fermentation.-Minimization and Prevention of Phage Infections in Bioprocesses.