Microbial Transposon Mutagenesis
Protocols and Applications
(Sprache: Englisch)
This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific...
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This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.
Inhaltsverzeichnis zu „Microbial Transposon Mutagenesis “
Part I: Transposon Protocols for Specific Microorganisms1. Preparation of Transposon Library and Tn-Seq Amplicon Library for Salmonella Typhimurium
Sardar Karash, Tieshan Jiang, Deepti Samarth, Reena Chandrashekar, and
Young Min Kwon
2. Application of Whole Genome Sequencing to Transposon Mutants of Salmonella Heidelberg
Bryna Rackerby, Sang In Lee, Ian Moppert, Steven C. Ricke, KwangCheol C. Jeong, and Si Hong Park
3. Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus
Jennifer L. Endres, Vijaya Kumar Yajjala, Paul D. Fey, and Kenneth W. Bayles
4. Transposon Mutagenesis in Streptococcus Species
Martin Nilsson, Michael Givskov, and Tim Tolker-Nielsen
5. Implementation of Transposon Mutagenesis in Bifidobacterium Lorena Ruiz and Douwe van Sinderen
6. Transposon Mutagenesis of Listeria monocytogenes
Oindrila Paul, Damayanti Chakravarty, and Janet R. Donaldson
7. Transposon Mutagenesis of Foodborne Pathogenic Esherichia coli
Supraja Puttamreddy and F. Chris Minion
8. Methods for Transposon Mutagenesis in Proteus mirabilis
Philip N. Rather
9. Mutagenesis of Vibrio fischeri and Other Marine Bacteria Using Hyperactive Mini-Tn5 Derivatives
Julie L. Stoudenmire, Michael Black, Pat M. Fidopiastis, and Eric V. Stabb
10. Transposon Mutagenesis of Bacteroides fragilis Yaligara Veeranagouda, Fasahath Husain, and Hannah M. Wexler
11. Transposon Mutagenesis in Mycobacterium avium Subspecies Paratuberculosis
John P. Bannantine, Denise K. Zinniel, and Raúl G. Barletta
Part II: Transposon General Protocols and Applications
12. Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches
Bijay K. Khajanchi, Pravin R. Kaldhone, and Steven L. Foley
13. Construction of DNA
... mehr
Barcode-Tagged Salmonella Strains
Yichao Yang, Reena Chandrashekar, Steven C. Ricke, and Young Min Kwon
14. Transposon-Aided Capture of Antibiotic Resistance Plasmids from Complex Samples
Sarah Delaney, Richard Murphy, and Fiona Walsh
15. Efficient Gene Deletion Method for Listeria monocytogenes
Hossam Abdelhamed, Attila Karsi, and Mark L. Lawrence
16. Whole Genome Identification and Characterization of Bacterial Insertion Sequences Using Bioinformatic Tools
Kody A. Bassett, Melanie R. Mormile, and Ronald L. Frank
Yichao Yang, Reena Chandrashekar, Steven C. Ricke, and Young Min Kwon
14. Transposon-Aided Capture of Antibiotic Resistance Plasmids from Complex Samples
Sarah Delaney, Richard Murphy, and Fiona Walsh
15. Efficient Gene Deletion Method for Listeria monocytogenes
Hossam Abdelhamed, Attila Karsi, and Mark L. Lawrence
16. Whole Genome Identification and Characterization of Bacterial Insertion Sequences Using Bioinformatic Tools
Kody A. Bassett, Melanie R. Mormile, and Ronald L. Frank
... weniger
Bibliographische Angaben
- 2019, 1st ed. 2019, XI, 183 Seiten, 34 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Steven C. Ricke, Si Hong Park, Morgan L. Davis
- Verlag: Springer, Berlin
- ISBN-10: 1493995693
- ISBN-13: 9781493995691
- Erscheinungsdatum: 14.06.2019
Sprache:
Englisch
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