miRNA Biogenesis
Methods and Protocols
(Sprache: Englisch)
This detailed volume collects chapters that seek to expand our knowledge of molecular events and extended molecular networks in gene regulation. The contents explore numerous aspects of miRNA biogenesis, a sophisticated series of events that assure a finely...
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Klappentext zu „miRNA Biogenesis “
This detailed volume collects chapters that seek to expand our knowledge of molecular events and extended molecular networks in gene regulation. The contents explore numerous aspects of miRNA biogenesis, a sophisticated series of events that assure a finely tuned regulation of miRNA expression and activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, miRNA Biogenesis: Methods and Protocols aims to contribute to the further development of knowledge and application of miRNA biogenesis, both in the basic research lab and in the clinic. ¿
Inhaltsverzeichnis zu „miRNA Biogenesis “
Chapter 1 microRNA biogenesis ReviewChapter 2 Identifying microRNA transcription sites
Chapter 3 Annotating primary microRNAs
Chapter 4 Defining Microprocessor co-factors
Chapter 5 Endogenous assay for primary microRNA processing
Chapter 6 In vitro assay for primary microRNA processing
Chapter 7 miRtrons
Chapter 8 Inhibiting primary microRNA processing
Chapter 9 Precursor microRNA characterization
Chapter 10 Precursor microRNA export
Chapter 11 Processing of precursor microRNAs in the cytoplasm
Chapter 12 Composition of the RNA-induced Silencing Complex
Chapter 13 RNA-sequencing of mature miRNAs
Chapter 14 qPCR measurement of mature microRNAs
Chapter 15 Stability of mature microRNAs
Chapter 16 MicroRNA modifications Chapter 17 MicroRNA tailing and trimming
Chapter 18 RNA modifications in microRNA biogenesis
Chapter 19 MicroRNA biogenesis in disease Case study
Bibliographische Angaben
- 2018, 1st ed. 2018, XI, 238 Seiten, 45 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Ulf Andersson Vang Ørom
- Verlag: Springer, Berlin
- ISBN-10: 1493986236
- ISBN-13: 9781493986231
- Erscheinungsdatum: 30.06.2018
Sprache:
Englisch
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