Molecular and Cellular Enzymology

Dynamics of Enzyme Reactions and Metabolic Networks in Living Cells. A Physico-Chemical Approach.- 1. Introduction.- 2. Flows and forces, diffusion, partition of mobile ions by charged matrices.- 3. Compartmentalization of enzyme reactions and the energy metabolism of the cell.- 4. Coupling between reactant diffusion and bound enzyme reaction rate.- 5. Electric partitioning of ions and reaction rate of bound enzyme systems.- 6. An example of enzyme behaviour in organized biological systems: the dynamics of enzymes bound to plant cell walls.- 7. Control and dynamics of enzyme networks.- 8. Control of multi-enzyme complexes.- 9. General Conclusions.- References.- Microbial and Genetic Approaches to the Study of Structure-Function Relationships of Proteins.- 1. Introduction.- 2. Protein-ligand and protein-substrate interactions.- 3. Protein evolution by point mutation.- 4. Protein evolution by rearrangements of combinatorial domains.- 5. Conclusion and perspectives.- References.- Recent Progress in Studies of Enzymatic Systems in Living Cells.- 1. Introduction: Why study the behaviour of enzymes in single living cells?.- 2. Tools available for studying the behavior of enzymes in single living cells.- 3. What next?.- 4. General conclusion.- References.