Mouse Cell Culture
Methods and Protocols
(Sprache: Englisch)
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers...
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Klappentext zu „Mouse Cell Culture “
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.
Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.
Inhaltsverzeichnis zu „Mouse Cell Culture “
1. The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid BodiesMelany Jackson, A. Helen Taylor, Elizabeth A. Jones, and Lesley M. Forrester2. Derivation of Primary Mouse Embryonic Fibroblast (PMEF) CulturesAlastair S. Garfield3. Embryonic Skeletal Muscle Micro-Explant Culture and Isolation of Skeletal Muscle Stem CellsJanet Smith and Deborah Merrick4. The Embryonic Kidney: Isolation, Organ Culture, Immunostaining, and RNA InterferenceJamie A. Davies5. Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular DifferentiationPierre-Marie del Moral and David Warburton6. Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and IntestineJonathan M. Quinlan, Wei-Yuan Yu, and David Tosh7. Isolation and Culture of Embryonic Pancreas and LiverZoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David Tosh8. Isolation and Culture of Mouse Satellite CellsAntonio Musarò and Laura Barberi9. Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte CulturesNigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and Paul J.R. Barton10. Short and Long-Term Cultivation of Embryonic and Neonatal Murine KeratinocytesReto Caldelari and Eliane J. Müller11. Isolation, Culture, and Analysis of Mouse Mammary Epithelial CellsMatthew J. Smalley12. Isolation and Culture of Mouse Pancreatic Islets for ex vivo Imaging Studies with Trappable or Recombinant Fluorescent ProbesMagalie A. Ravier and Guy A. Rutter13. Isolation and Culture of Adult Mouse HepatocytesWan-ChunLi, Kate L. Ralphs, and David Tosh14. Isolation and Culture of Mouse Intestinal CellsF. Charles Campbell15. Derivation of Primary Choroid Plexus Epithelial Cells from the MouseTrevelyan R. Menheniott, Marika Charalambous, and Andrew Ward16. The Preparation of Primary Cortical Neuron Cultures and a
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Practical Application Using Immunofluorescent CytochemistryCarla Sciarretta and Liliana Minichiello17. Cell Culture of Primary Cerebellar Granule CellsDana Krämer and Liliana Minichiello18. Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse BrainHenrik Ahlenius and Zaal Kokaia
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Bibliographische Angaben
- 2010, 272 Seiten, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: David Tosh, Andrew Ward
- Verlag: Humana Press
- ISBN-10: 1588297721
- ISBN-13: 9781588297723
- Erscheinungsdatum: 11.03.2010
Sprache:
Englisch
Rezension zu „Mouse Cell Culture “
From the reviews:"This book provides a diverse collection of protocols for mouse cell culture in a text comprising 18 chapters. ... This book is recommended for researchers and students who are interested in studying the culture of cells from animal models." (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010)
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