Mouse Cell Culture

The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies.- Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures.- Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells.- The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference.- Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation.- Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine.- Isolation and Culture of Embryonic Pancreas and Liver.- Isolation and Culture of Mouse Satellite Cells.- Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures.- Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes.- Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells.- Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes.- Isolation and Culture of Adult Mouse Hepatocytes.- Isolation and Culture of Mouse Intestinal Cells.- Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse.- The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry.- Cell Culture of Primary Cerebellar Granule Cells.- Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain.

From the reviews:"This book provides a diverse collection of protocols for mouse cell culture in a text comprising 18 chapters. ... This book is recommended for researchers and students who are interested in studying the culture of cells from animal models." (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010)