NF- B Transcription Factors

Measuring NF-kB Phosphorylation and Acetylation.- Biochemical Methods to Analyze the Subcellular Localization of NF- B Proteins Using Cell Fractionation.- Immunohistochemical Analysis of Expression, Phosphorylation, and Nuclear Translocation of NF- B Proteins in Human Tissues.- High-Throughput Analysis of the Cell- and DNA Site-Specific Binding of Native NF- B Dimers Using Nuclear Extract Protein-Binding Microarrays (NextPBMs).- Molecular and Biochemical Approaches to Study the Evolution of NF- B Signaling in Basal Metazoans.- Methods for Modulating the Pathway of NF-kB Using Short Hairpin RNA (ShRNA).- Immunoblot Analysis of the Regulation of TNF Receptor Family-Induced NF-kB Signaling by c-IAP Proteins.- Methods to Study CARD11-BCL10-MALT1 Dependent Canonical NF-kB Activation in Jurkat T-Cells.- Analysis of Calcium Control of Canonical NF- B Signaling in B Lymphocytes.- A Kinase Assay for Measuring the Activity of the NIK-IKK1 Complex Induced via the Noncanonical NF- B Pathway.- Analyze the SUMOylation of IKKGamma/NEMO During Genotoxic Stress.- Analysis of the Contribution of NF-kB in the Regulation of Chemotherapy-Induced Cell Senescence by Establishing a Tetracycline-Regulated Cell System.- Methods to Detect NF- B Activity in Tumor-Associated Macrophage (TAM) Populations.- Methods to Study the Effect of IKK Inhibition on TNF-Inducing Apoptosis and Necroptosis in Cultured Cells.- Use of ChIP-qPCR to Study the Crosstalk between HIF and NF-kB Signaling in Hypoxia and Normoxia.- Methods to Analyze the Roles of TAK1, TRAF6, and NEMO in the Regulation of NF- B Signaling by RANK Stimulation During Osteoclastogenesis.- NF-kB Signaling in Ex Vivo Mouse Intestinal Organoids.- Extracellular Flux Analysis to Investigate the Impact of NF- B on Mitochondrial Respiration in Colorectal Carcinoma (CRC).- Conditional Knockout Mouse Models to Study the Roles of Individual NF- B Transcription Factors in Lymphocytes.- Generation and Surgical Analysis of Genetic