Notch Signaling

1. Introduction to Notch Signaling

Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen

2. Genetic Screens to Identify New Notch Pathway Mutants in Drosophila

Nikolaos Giagtzoglou

3. Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes

Jessica Leonardi and Hamed Jafar-Nejad

4. Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice

Thomas Gridley and Andrew K. Groves

5. Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster

Emiliana Tognon and Thomas Vaccari

6. Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila

Lydie Couturier and François Schweisguth

7. Tracking Trafficking of Notch and its Ligands in Mammalian Cells

Patricia Chastagner and Christel Brou

8. Visualizing Notch Signaling in vivo in Drosophila Tissues

Benjamin E. Housden, Jinghua Li, and Sarah J. Bray

9. Monitoring Notch Activity in the Mouse

Lavinia Alberi and Swananda Marathe

10. Notch Signaling Assays in Drosophila Cultured Cell Lines

Jinghua Li, Benjamin E. Housden, and Sarah J. Bray

11. Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays

Ma. Xenia G. Ilagan and Raphael Kopan

12. Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays

Ma. Xenia G. Ilagan and Raphael Kopan

13. Visualization of Notch Signaling Oscillation in Cells and Tissues

Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama

14. Proteomic Analysis of the Notch Interactome

K. G. Guruharsha, Kazuya Hori, Robert A. Obar, and Spyros Artavanis-Tsakonas

15. Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands

Pat Whiteman, Christina Redfield and Penny A. Handford

16. Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry

Shinako Kakuda and Robert S.