Oligodendrocytes
Methods and Protocols
(Sprache: Englisch)
This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular...
Jetzt vorbestellen
versandkostenfrei
Buch (Gebunden)
186.99 €
- Lastschrift, Kreditkarte, Paypal, Rechnung
- Kostenlose Rücksendung
- Ratenzahlung möglich
Produktdetails
Produktinformationen zu „Oligodendrocytes “
Klappentext zu „Oligodendrocytes “
This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.
Inhaltsverzeichnis zu „Oligodendrocytes “
Preface...Table of Contents...Contributing Authors... 1. Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous SystemSueli Marques, David van Bruggen, and Gonçalo Castelo-Branco 2. Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA SequencingClaire L. Davies, Stefka Gyoneva, Anne Cotleur, Richard M. Ransohoff, and Veronique E. Miron 3. Myelin: Methods for Purification and Proteome AnalysisMichelle S. Erwig, Dörte Hesse, Ramona B. Jung, Marina Uecker, Kathrin Kusch, Stefan Tenzer, Olaf Jahn, and Hauke B. Werner 4. In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem CellsDario Magnani, Siddharthan Chandran, David J.A. Wyllie, and Matthew R. Livesey 5. Isolation and Culture of OligodendrocytesMarie-Theres Weil, Giselheid Schulz-Ëberlin, Chaitali Mukherjee, Wen Ping Kuo-Elsner, Isabelle Schäfer, Christina Müller, and Mikael Simons 6. A Neuron-Free Microfiber Assay to Assess Myelin Sheath FormationMarie E. Bechler 7. Oligodendrocyte-Neuron Myelinating Co-CultureMatthew Swire and Chalres ffrench-Constant 8. Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of RanvierAndrew A. Jarjour and Diane L. Sherman 9. Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain SlicesSylvia Agathou and Ragnhildur Thóra Káradóttir 10. Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal CordSowmya Sekiza and Anna Williams 11. Forward Genetic Screen using Zebrafish to Identify New Genes Involved in MyelinationLinde Kegel, Maria Rubio, Rafael Almeida, Silvia Benito, Anna Klingseisen, and David A. Lyons 12. Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive MyelinationJill M. Williamson, David A. Lyons, and Rafael G. Almeida 13. A Drug Inducible Transgenic Zebrafish Model for
... mehr
Myelinating Glial Cell AblationMarja J. Karttunen and David A. Lyons 14. Conditional Demyelination and Remyelination in a Transgenic Xenopus laevisAbdelkrim Mannioui and Barnard zalc 15. Conditional Mutagenesis in Oligodendrocyte Lineage CellsSandra Goebbels and Klaus-Armin Nave 16. Recent Advances in Live Imaging of Cells of the Oligodendrocyte LineageJaime Eugenin-von Bernhardi and Leda Dimou 17. The DTA Mouse Model for Oligodentrocyte Ablation and CNS DemyelinationMaria Traka 18. Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human DiseaseJohn N. Mariani, Lisa Zou, and Steven A. Goldman 19. A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in OligodencrocytesGraham R. Campbell and Don J. Mahad 20. Transmission Electron Microscopy of Oligodendrocytes and MyelinMarie-Theres Weil, Torben Ruhwedel, Martin Meschkat, Mogusia Sadowski, and Wiebke Möbius 21. Toxin-Based Models to Investigate Demyelination and RemyelinationChristopher E. McMurran, Chao Zhao, and Robin J M. Franklin 22. Magnetic Resonance techniques for Imaging White MatterCassandra Sampaio-Baptista, Kata Diosi, and Heidi Johansen-Berg
... weniger
Bibliographische Angaben
- 2019, 1st ed. 2019, XII, 411 Seiten, 55 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: David A. Lyons, Linde Kegel
- Verlag: Springer, Berlin
- ISBN-10: 1493990705
- ISBN-13: 9781493990702
- Erscheinungsdatum: 01.03.2019
Sprache:
Englisch
Kommentar zu "Oligodendrocytes"
Schreiben Sie einen Kommentar zu "Oligodendrocytes".
Kommentar verfassen